Evaluation of a povidone-iodine and chitosan-based barrier teat dip in the prevention of mastitis in dairy cows  

作　　者：HANG Hui-min JIANG Hong-rui CHEN Dai-jie SHEN Zi-liang MAO Yong-jiang LIANG Yusheng Juan JLOOR YANG Zhang-ping 

机构地区：[1]Key Laboratory for Animal Genetics,Breeding,Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,P.R.China [2]Joint International Research Laboratory of Agriculture&Agri-Product Safety,Ministry of Education/Yangzhou University,Yangzhou 225009,P.R.China [3]Department of Animal Sciences&Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA

出　　处：《Journal of Integrative Agriculture》2021年第6期1615-1625,共11页农业科学学报（英文版）

基　　金：the National Natural Science Foundation of China(31872324,31702142);the Jiangsu Modern Dairy Industry Technology System,China(JATS(2018)300)。

摘　　要：Postmilking teat dip is an important tool used to prevent mastitis in the modern dairy industry.In this study,we evaluated the in vitro and in vivo efficacies of a barrier teat dip containing povidone-iodine and chitosan for the prevention of mastitis.In experiment 1,we evaluated the antibacterial effects of chitosans with different molecular weights against six mastitis-causing bacteria based on the minimal inhibitory concentration test.The results showed that 50 k Da chitosan had the maximum antibacterial activity compared with 5,150 and 350 k Da chitosans.In experiment 2,the inhibition zone test indicated that the barrier teat dip with 4.0%povidone-iodine and 1.0%chitosan had higher(P<0.05)in vitro antibacterial efficacy against most tested mastitis-causing bacteria than the barrier teat dip with 4.0%povidone-iodine and no chitosan.In experiments 3 and 4,we evaluated the efficacies of two postmilking teat dips,1)a barrier teat dip containing 1.0%chitosan and 4.0%povidone-iodine and 2)a conventional nonbarrier product containing 10%povidone-iodine in a field trial at two commercial dairy herds(1 and 2).A 56-d split-udder experiment(experiment 3)was conducted using 47 lactating Chinese Holstein cows in herd 1.Both left teats were immersed in barrier postmilking dip,and both right teats were dipped with nonbarrier postmilking dip.During a 56-d split-herd experiment(experiment 4),a total of 139 lactating Chinese Holstein cows from herd 2 were allocated to two groups:1)all teats of 67 cows were dipped in the nonbarrier teat dip,and 2)all teats of 72 cows were dipped in the barrier teat dip.Milk samples were collected and analyzed for somatic cell count(SCC),fat content,protein content,and fat-to-protein ratio prior to the start of sampling(0 d),and at 28 and 56 d after initiation.Bacteriological analysis was only performed on milk samples with SCC≥200000 cells mL^(-1).In experiment 3,no differences(P>0.05)in SCC,somatic cell score(SCS)or other milk quality indicators were observed between nonbarrier and barrier t

关 键 词：MASTITIS POVIDONE-IODINE CHITOSAN barrier postmilking dip 

分 类 号：S858.23[农业科学—临床兽医学]