萱草花粉电击转化参数的选择  

Selection of pollen electroporation transformation parameters of daylily(Hemerocallis sp.)

作　　者：钟荣 谭雨馨 张铮 尹冬梅 刘翔 秦巧平 张志国 倪迪安 ZHONG Rong;TAN Yuxin;ZHANG Zheng;YIN Dongmei;LIU Xiang;QIN Qiaoping;ZHANG Zhiguo;NI Di’an(School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学生态技术与工程学院,上海201418

出　　处：《植物生理学报》2021年第3期723-726,共4页Plant Physiology Journal

基　　金：上海市科技兴农项目[沪农科推字(2019)第1-8号]。

摘　　要：以萱草品种‘斯特拉’成熟花粉为材料,比较了不同电场强度和电击次数对花粉转化率的影响。结果显示:萱草花粉电击转化的最佳参数为1 kV·cm^(-1)、电击3次。Different pulse field strength and number of pulses were used for electroporation transformation of mature pollens of daylily(Hemerocallis sp.) ’Black Eyed Stella’ to obtain an optimal parameter. The results showed that the optimal parameter of daylily pollen electroporation was 1 kV·cm^(-1) with 3 pulses.

关 键 词：萱草 花粉 电击转化 

分 类 号：S682.19[农业科学—观赏园艺]