Nanograins on Ti-25Nb-3Mo-2Sn-3Zr alloy facilitate fabricating biological surface through dual-ion implantation to concurrently modulate the osteogenic functions of mesenchymal stem cells and kill bacteria  

作　　者：Run Huang Lei Liu Bo Li Liang Qin Lei Huang Kelvin W.K.Yeung Yong Han 

机构地区：[1]School of Materials Science and Engineering,Anhui University of Science and Technology,Huainan,232001,China [2]Department of Orthopaedics and Traumatology,The University of Hong Kong,Hong Kong,China [3]State-key Laboratory for Mechanical Behavior of Materials,Xi’an Jiaotong University,Xi'an,710049,China [4]Department of Gastrointestinal Surgery,Hubei Cancer Hospital,Wuhan,430060,China [5]Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma,The University of Hong Kong Shenzhen Hospital,Shenzhen,518053,China

出　　处：《Journal of Materials Science & Technology》2021年第14期31-44,共14页材料科学技术（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.81501598,51631007 and 31700860);the China Postdoctoral Science Foundation(No.2017M612052);the Postdoctoral Foundation of Anhui Province(No.2017B211);the General Research Fund of Research Grant Council of Hong Kong(Nos.N HKU725-16,17207719 and 17214516)。

摘　　要：Surface mechanical attrition treatment(SMAT)method is an effective way to generate nanograined(NG)surface on Ti-25 Nb-3 Mo-2 Sn-3 Zr(wt.%)(named as TLM),a kind ofβ-type titanium alloy,and the achieved nanocrystalline surface was proved to promote positive functions of osteoblastic cells.In this work,to further endow the NG TLM alloy with both good osteogenic and antibacterial properties,magnesium(Mg),silver(Ag)ion or both were introduced onto the NG TLM surface by ion implantation process,as a comparison,the Mg and Ag ions were also co-implanted onto coarsegrained(CG)TLM surface.The obtained results show that subsequent ion implantation does not remarkably induce the surface roughness and topography alteration of the SMAT-treated layers,and it also has little impact on the microstructure of the SMAT-derivedβ-Ti nanograins.In addition,the implanted Mg and Ag ions are observed to exist as MgO and metallic Ag na noparticles(NPs)embedding tightly in theβ-Ti matrix with grain size of about 15 and 7 nm,respectively.Initial cell adhesion and functions(including proliferation,osteo-differentiation and extracellular matrix mineralization)of rabbit bone marrow mesenchymal stem cells(rBMMSCs)and the bacterial colonization of Staphylococcus aureus(S.aureus)on the different surfaces were investigated.The in-vitro experimental results reveal that the Mg and Ag single-ion implanted NG surface either significantly promotes the rBMMSCs response or inhibits the growth ofS.aureus,whereas the Mg/Ag coimplanted NG surface could concurrently enhance the rBMMSCs functions as well as inhibit the bacterial growth compared to the NG surface,and this efficacy is more pronounced as compared to the Mg/Ag co-implantation in the CG surface.The SMAT-achieved nanograins in the TLM surface layer are identified to not only play a leading role in determining the fate of rBMMSCs but also facilitate fabricating dualfunctio nal surface with both good osteogenic and antibacterial activities through co-implantation of Mg and Ag ions.Our investigation

关 键 词：β-Type titanium Nanograined surface Ion implantation Stem cells Antibacterial property 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]