GGTA1基因敲除猪胰岛细胞移植到Ⅰ型糖尿病猕猴3例报道  

Report of 3 cases of transplantation of GGTA1 gene knockout porcine islet cells into typeⅠdiabetic macaques

作　　者：谢雨宣 唐红梅 李欣 潘登科 周佳 XIE Yuxuan;TANG Hongmei;LI Xin;PAN Dengke;ZHOU Jia(Institute of Organ Transplantation,Sichuan Academy of Medical Sciences,Sichuan Provincial People’s Hospital,Chengdu 610072,P.R.China;University of Electronic Science and Technology of China School of Medicine,Chengdu 610054,P.R.China;Institute of Laboratory Animal Sciences,Sichuan Academy of Medical Sciences,Sichuan Provincial People’s Hospital,Chengdu 610212,P.R.China)

机构地区：[1]四川省医学科学院·四川省人民医院器官移植研究所,成都610072 [2]电子科技大学医学院,成都610054 [3]四川省医学科学院·四川省人民医院实验动物研究所,成都610212

出　　处：《中国普外基础与临床杂志》2021年第5期561-565,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：四川省科技计划资助(项目编号:2021YFS0008)。

摘　　要：目的探索GGTA1基因敲除猪胰岛细胞移植到Ⅰ型糖尿病猴的效果。方法本研究采用GGTA1基因敲除新生猪胰岛细胞开展了3例猪-猴异种胰岛移植实验。结果3只猕猴成功进行胰岛细胞移植治疗,术后生命体征平稳,未发生静脉栓塞症状。移植后血糖和外源胰岛素用量均显著降低,能检测到特异性猪C肽。移植后3只猕猴均发生了酮症酸中毒症状,1只猕猴发生伤口感染,经对症处理后均健康存活达16周。结论GGTA1基因敲除新生猪胰岛细胞经肝门静脉移植治疗1型糖尿病的方法是有效的。Objective To explore the effect of transplanting neonatal porcine islet cells of pig via hepatic portal vein in typeⅠdiabetic monkeys.Method In this study,three pig-monkey islet xenotransplantation experiments were carried out by using α-1,3-galactosyltransferase(GGTA1)gene knockout neonatal pig islet cells.Results Three macaques were successfully transplanted with islet cells.After the operation,their vital signs were stable and no symptoms of venous embolism occurred.After transplantation,the blood glucose and the dosage of exogenous insulin were significantly reduced,and the specific porcine C-peptide could be detected.Three macaques developed symptoms of ketoacidosis,and one macaque developed wound infection.After symptomatic treatment,all of them survived for 16 weeks.Conclusion GGTA1 knockout neonatal porcine islet cells transplanted through hepatic portal vein is effective for the treatment of type Ⅰ diabetes.

关 键 词：猪-猴异种胰岛移植 α-1 3-半乳糖基转移酶基因 基因敲除 1型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]