电针联合骨髓间充质干细胞注射对大鼠薄型子宫内膜雌、孕激素受体的影响  

Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats

作　　者：孟庆宇 席瑾 夏良君 杨滨 程洁[1] 夏有兵 万茜[4] MENG Qing-yu;XI Jin;XIA Liang-jun;YANG Bin;CHENG Jie;XIA You-bing;Wan Qian(College of Acupuncture,Massage,Health and Rehabilitation,Nanjing University of Chinese Medicine,Nanjing 210046,China;College of Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing 210046;Xuzhou Medical University,Xuzhou 221004,Jiangsu Province;Department of Traditional Chinese Medicine,Gulou Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210046)

机构地区：[1]南京中医药大学针灸推拿·养生康复学院,南京210046 [2]南京中医药大学医学院·整合医学院,南京210046 [3]徐州医科大学,江苏徐州221004 [4]南京大学医学院附属鼓楼医院中医科,南京210046

出　　处：《针刺研究》2021年第5期385-390,共6页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81873371)。

摘　　要：目的:观察电针联合骨髓间充质干细胞注射对大鼠薄型子宫内膜雌、孕激素受体的影响。方法:SD大鼠分为空白组、模型组、细胞组、联合组,每组10只。用95%乙醇宫腔注射法复制薄型子宫内膜大鼠模型。细胞组与联合组于造模当日、造模后第3天、第2个动情期、第3个动情期当日由尾静脉进行干细胞悬液注射。联合组除干细胞注射外,予电针"关元""子宫""三阴交",每日1次,连续10 d。3个动情周期后的动情期取子宫标本,计算子宫系数,免疫组织化学法检测子宫组织KI67表达,Western blot法检测子宫组织雌激素受体、孕激素受体的表达。结果:与空白组比较,模型组子宫系数及雌激素受体、孕激素受体表达量均显著降低(P<0.001,P<0.01)。与模型组比较,细胞组、联合组子宫系数及KI67、雌激素受体、孕激素受体表达量均明显升高(P<0.000 1,P<0.001,P<0.01)。联合组孕激素表达量比细胞组升高(P<0.05)。结论:电针联合骨髓间充质干细胞注射可促进大鼠薄型子宫内膜细胞增殖,升高雌、孕激素受体水平。Objective To investigate the effect of joint administration of electroacupuncture(EA) and bone marrow mesenchymal stem cells(BMMSC) on the expression of estrogen receptor(ER) and progesterone receptor(PR) in thin endometrium rats, so as to explore its mechanisms underlying improvement of conception rate. Methods Forty female SD rats were divided into control, model, BMMSC and EA+BMMSC groups. The thin endometrium model was established by intrauterine infusion of 95% ethanol and saline. EA(1 mA,2 Hz/15 Hz) was applied to "Guanyuan"(CV4), unilateral "Zigong"(EX-CA1) and unila-teral "Sanyinjiao"(SP6) for 15 min, once daily for 10 d. Rats of the BMMSC and EA+BMMSC groups received injection of BMMSC suspension through caudal vein on the day of modeling, the 3rd day after surgery, and the 2nd and the 3rd estrus phases, respectively. Changes of body weight and estrus phase were continuously recorded. After three estrus cycles, uterine specimens were taken and the uterine coefficient was calculated. The immunoactivity of KI67(an antigen for cell proliferation) was detected by immunohistochemistry, and the expression of ER and PR was detected by Western blot. Results Compared with the control group, the uterine coefficient, and expression of endometrium ER and PR proteins were significantly decreased in the model group(P<0.001,P<0.01). In Comparison with the model group, the uterine coefficient, immunoactivity of KI67, and expression of ER and PR proteins were significantly increased in both BMMSC and EA+BMMSC groups(P<0.000 1,P<0.001, P<0.01). The expression level of PR in the EA+BMMSC group was notably higher than that in the BMMSC group(P<0.05), but no significant difference was found between the BMMSC and EA+BMMSC groups in the levels of uterine coefficient and ER expression(P>0.05). Conclusion EA combined with BMMSC injection has a positive effect in promoting the proliferation of endometrium cells in rats with thin endometrium, which may be related to its effect in up-regulating the expression of ER and PR proteins.

关 键 词：电针 薄子宫内膜 骨髓间充质干细胞 子宫内膜细胞增殖 KI67 雌激素受体 孕激素受体 

分 类 号：R245.97[医药卫生—针灸推拿学]