电针对肌萎缩侧索硬化小鼠疾病进程及腰髓热休克蛋白70表达的影响  

Effect of electroacupuncture on expression of heat shock protein 70 in mice with amyotrophic lateral sclerosis

作　　者：刘霞蔚 闫英 黑伊凡 朱晨军 田青 孙文军 LIU Xia-wei;YAN Ying;HEI Yi-fan;ZHU Chen-jun;TIAN Qing;SUN Wen-jun(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《针刺研究》2021年第5期391-396,共6页Acupuncture Research

基　　金：北京中医药大学重点攻关课题(No.2020-JYB-ZDGG-144-2);北京中医药大学自主课题青年教师项目(No.2018-JYBZZ-JS 178)。

摘　　要：目的:观察电针对肌萎缩侧索硬化(ALS)小鼠体质量、瘫痪时间、生存期及腰髓热休克蛋白70(HSP70)表达的影响,从营养状况和能量代谢角度探讨电针治疗ALS的机制。方法:将18只ALS转基因SOD1G93A小鼠随机分为模型组、电针组和西药组,每组6只,以6只C57BL/6J小鼠作为正常组。电针组予电针"曲池""合谷""足三里""三阴交",30 min/次,5次/周,共8周;西药组予利鲁唑溶液(7.6 mg·kg^(-1)·d^(-1))灌胃,连续8周。每周固定时间称量小鼠体质量;采用ALS治疗发展研究所神经功能评分标准评估小鼠后肢运动功能;采用Western blot法和免疫组织化学法分别检测腰髓组织HSP70蛋白的表达。结果:与正常组比较,模型组小鼠体质量、HSP70蛋白表达显著降低(P<0.05);与模型组比较,电针组和西药组小鼠的发病时间和瘫痪时间均显著推迟(P<0.05,P<0.01),HSP70蛋白表达显著升高(P<0.05,P<0.01);与电针组比较,西药组小鼠的发病时间显著推迟(P<0.05)。结论:电针干预能增加小鼠腰髓HSP70的表达,延缓ALS疾病进程。Objective To observe the effects of electroacupuncture(EA) on the body weight, disease progression and the expression of heat shock protein 70(HSP70) in lumbar spinal cord of amyotrophic lateral sclerosis(ALS) mice, so as to explore the mechanism of EA on treating ALS. Methods Eighteen ALS transgenic SOD1G93 A mice were randomly divided into model, EA and medication groups, with 6 mice in each group, and six C57 BL/6 J mice were used as the normal group. Mice in the EA group received EA at "Quchi"(LI11)-"Hegu"(LI4), "Zusanli"(ST36)-"Sanyinjiao"(SP6), 30 min/time, 5 times/week, for 8 weeks.Mice in the medication group were given riluzole solution(7.6 mg·kg^(-1)·d^(-1)) by gavage for 8 weeks. The body weight of the mice was recorded and the motor function of the hind limbs was evaluated by the neurological function scoring stan-dard of the ALS Therapeutic Development Institute. The expression of HSP70 in lumbar spinal cord was detected by Western blot and immunohistochemistry, respectively. Results Compared with the normal group, the body weight and the expression of HSP70 in the model group decreased significantly(P<0.05), while no significant difference in the body weight was found among other groups(P>0.05). After intervention and in comparison with the model group, the disease onset time and paralysis time of the EA group and the medication group were significantly delayed(P<0.05,P<0.01), the expression of HSP70 in the EA group and the medicine group was significantly increased(P<0.05,P<0.01).But there was no significant difference in the survival time among all groups(P>0.05). The disease onset time of the EA group was shorter than that in the medication group(P<0.05).Conclusion EA can increase the expression of HSP70 in lumbar spinal cord, thereby delaying the progression of ALS.

关 键 词：电针 肌萎缩侧索硬化 转基因小鼠 热休克蛋白70 能量代谢 

分 类 号：R245.97[医药卫生—针灸推拿学]