A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment  

作　　者：Kun-Yang Wang Jie Shao Li-Gang Shao Jia-Jin Chen Gui-Shi Wang Kun Liu Xiao-Ming Gao 王坤阳;邵杰;邵李刚;陈家金;王贵师;刘琨;高晓明(Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Science,Hefei 230031,China;University of Science and Technology of China,Hefei 230031,China;College of Physics and Electronic Information Engineering,Zhejiang Normal University,Zhejiang 321004,China)

机构地区：[1]Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Science,Hefei 230031,China [2]University of Science and Technology of China,Hefei 230031,China [3]College of Physics and Electronic Information Engineering,Zhejiang Normal University,Zhejiang 321004,China

出　　处：《Chinese Physics B》2021年第5期378-383,共6页中国物理B（英文版）

基　　金：the National Key Research and Development Program of China(Grant Nos.2017YFC0209700 and 2016YFC0303900);the National Natural Science Foundation of China(Grant No.41730103).

摘　　要：A new pressure-calibration method for calibrating the reduction of second harmonic(2f)amplitude caused by pressure broadening effect in sealed microbial growth environment is present.The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO_(2) in microbial growth.In order to verify the method,a simulation experiment is developed,in which the increasing CO_(2) concentration leads to the increasing pressure.Comparing with the relation between the traditional 2f amplitude and gas concentration,there is a monotonous relation between the calibrated 2f amplitude and CO_(2) concentration,particularly,a linear relation is present when the CO_(2) concentration is replaced with the CO_(2) particle number.In terms of microbial measurement,the growth of Escherichia coli is measured,and the culture bottle is sealed during the microbial growth process.The experimental results show that,comparing to the microbial growth retrieved by traditional 2f amplitude,the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.

关 键 词：second harmonic amplitude pressure-calibration microbial growth sealed environment CO_(2) 

分 类 号：Q93-33[生物学—微生物学]