甘蔗‘桂热2号’和‘桂柳05136’成熟期转录组比较分析  

Comparative Transcriptome Analysis of ‘GR2’ and ‘GL05136’ in Mature-stage of Sugarcane

作　　者：卢业飞[1] 欧克纬[1] 罗清[1] 张宇[1] 程琴[1] 朱鹏锦[1] 周全光[1] 庞新华[1] 吕平[1] Lu Yefei;Ou Kewei;Luo Qing;Zhang Yu;Cheng Qin;Zhu Pengjin;Zhou Quanguang;Pang Xinhua;Lü Ping(Guangxi Subtropical Crops Research Institute,Nanning,530001)

机构地区：[1]广西壮族自治区亚热带作物研究所,南宁530001

出　　处：《分子植物育种》2021年第9期2846-2855,共10页Molecular Plant Breeding

基　　金：广西农科院科技发展基金项目(桂农科2020YM137);广西创新驱动发展专项资金项目(桂科AA17202042-5);广西科学研究与技术开发计划项目(桂科攻1598006-1-1B)共同资助。

摘　　要：‘桂热2号’和‘桂柳05136’分别属于晚熟和早熟的甘蔗高糖新品种。对这两个品种的叶片和茎秆组织混样进行转录组测序分析,比较基因表达差异性,筛选和克隆可能参与糖分合成的相关基因。采用相同时期的种间对比和不同时期的种内对比两种方式,设置4个对比组,着重以KEGG和GO数据库分析转录组总体以及在糖分合成相关领域的基因差异表达情况。结果发现与糖分合成相关的5种酶基因序列97条及功能未知的多个高表达基因,后续可以通过构建转基因甘蔗验证体系验证高表达基因的功能,完善糖分代谢的分子机制理论,开发高糖分子标记甚至是构建高表达转基因品种。Sugarcane‘GR2’and ‘GL05136’ belong to late and early maturing high sugar varieties.Transcriptome sequencing was performed on the mixed samples of the leaves and stems of the two varieties to compare the differences in gene expression and to screen and clone genes that may be involved in sugar synthesis.Two comparison methods of interspecific at the same period and intraspecific at different periods were adopted,and four comparison groups were set up in the two varieties.KEGG and GO databases were used to analyze the overall transcriptome and the gene differential expression in the related fields of sugar synthesis.The results showed that there were 97 gene sequences of 5 enzymes related to sugar synthesis and several high-expression genes whose functions were unknown.Subsequently,the function of the high-expression genes could be verified by building a transgenic sugarcane validation system,improving the molecular mechanism theory of sugar metabolism,developing high-sugar molecular markers and even building high-expression transgenic varieties.

关 键 词：甘蔗 糖分合成 转录组 ‘桂热2号’ ‘桂柳05136’ 

分 类 号：S566.1[农业科学—作物学]