植物生长调节剂对杜鹃兰种胚发育的影响  

Effects of Plant Growth Regulators for Seed Embryos Development of Cremastra appendiculata

作　　者：田莉 高燕燕[1] 杨宁线 彭思静 张明生[1] Tian Li;Gao Yanyan;Yang Ningxian;Peng Sijing;Zhang Mingsheng(School of Life Science,Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学生命科学学院,山地植物资源保护与种质创新教育部重点实验室,贵阳550025

出　　处：《分子植物育种》2021年第9期3090-3095,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(81660627;81360613);国家重点研发计划课题(2016YFC0502604);贵州省科技计划项目(黔科合平台人才[2017]5411-06;黔科合平台人才[2017]5788号);贵州省科技创新人才团队建设专项资金项目(黔科合平台人才[2016]5624);贵州省高层次创新型人才培养计划项目(黔科合人才[2015]4031号);贵州省教育厅创新群体重大研究项目(黔教合KY字[2016]023)共同资助。

摘　　要：为了筛选获得最佳植物生长调节剂配比以实现杜鹃兰种胚快速发育成熟,本试验以杜鹃兰种子为实验材料,研究了不同浓度萘乙酸(NAA)、6-苄氨基嘌呤(6-BA)、激动素(KT)、玉米素(ZT)、吲哚丁酸(IBA)、2,4-二氯苯氧乙酸(2,4-D)对杜鹃兰种胚发育的影响。结果表明:在培养基中添加六种植物生长调节剂对杜鹃兰种胚发育均有不同程度的影响。2,4-D抑制胚的发育;添加KT与ZT,40 d后种子才开始吸水膨胀,胚发育缓慢;NAA、IBA、6-BA均能加快胚的生长,单独添加时,NAA促进种胚发育效果最好,其中NAA与6-BA配合使用且NAA∶6-BA=1 mol/L∶1 mol/L时胚发育速度明显高于对照及其他配比组合,40 d种子萌发率达88.11%。本研究为植物生长调节剂促进杜鹃兰种胚发育提供了很好的理论依据,有利于促进杜鹃兰种胚发育研究领域空白,以期为构建杜鹃兰种苗快繁体系发展。In order to understand the process of embryo development of Rhododendron seed embryo under different plant growth regulators,the seed of Rhododendron was used as the experimental material for the rapid development and maturation of Cremastra appendiculata seeds embryo.Using Cremastra appendiculata seeds as experimental materials,the plant growth regulators naphthaleneacetate(NAA),6-benzylaminopurine(6-BA),kinetin(KT),zeatin(ZT),beta-indolebutyric acid(IBA),effects of 2,4-dichlorophenoxyacetic acid(2,4-D)on the development of azalea seed embryos.The results showed that the addition of six plant growth regulators to the medium had different effects on the development of azalea seed embryos.2,4-D inhibited the development of embryo;Adding KT and ZT,the seeds began to expand after 40 days,and the development of embryo was slow;NAA,IBA and 6-BA could accelerate the growth of embryo,and NAA had the best effect on promoting the development of embryo when it was added alone,in which NAA and 6-BA were used together and NAA∶6-BA=1 mol/L∶1 mol/L,the speed of embryo development was the fastest,and the germination rate of seed reached 88.11%after 40 days.Therefore,it is necessary to fill the blank in the field of embryo development of Rhododendron so as to provide theoretical basis for the construction of rapid propagation system of Cremastra appendiculata seedlings.

关 键 词：杜鹃兰(Cremastra appendiculata) 种胚发育 植物生长调节剂 

分 类 号：S682.31[农业科学—观赏园艺]