雪莲细胞培养物的质量标准研究  

Study on Quality Standard of Cell Cultures of Saussurea involucrata

作　　者：张敏[1] 张雪松 刘雅萍 李晓琳[1] 南铁贵[1] 袁媛[1,2] ZHANG Min;ZHANG Xuesong;LIU Yaping;LI Xiaolin;NAN Tiegui;YUAN Yuan(National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;National and Local Joint Engineering Research Center for Rare and Endangered Medicinal Plants,Dalian 116001,China)

机构地区：[1]中国中医科学院中药资源中心,北京100700 [2]珍稀濒危药用植物国家地方联合工程研究中心,大连116001

出　　处：《世界中医药》2021年第7期1013-1017,1030,共6页World Chinese Medicine

基　　金：国家科技基础性资源调查专项(2018FY100802);国家自然科学基金重大项目(81891013,81891010);国家重点研发计划项目(2020YFE0205100);中央级公益性科研院所基本科研业务费专项资金资助项目(ZZ10-008)。

摘　　要：目的:建立雪莲细胞培养物的质量标准。方法:参照《中华人民共和国药典》2020年版附录,分别建立雪莲细胞培养物的一般检查项目及定性定量指标。以芦丁为对照品,利用紫外分光光度法建立雪莲细胞培养物总黄酮成分含量的测定方法;利用高效液相色谱(HPLC)法同时测定绿原酸、紫丁香苷和1,5-二咖啡酰奎尼酸成分含量,并建立其特征图谱,采用2012版《中药色谱指纹图谱相似度评价系统》对特征图谱进行相似度分析。结果:15批次雪莲细胞培养物总黄酮类成分含量10.0%~11.0%;绿原酸含量0.86%~1.09%;紫丁香苷含量0.34%~0.48%;1,5-二咖啡酰奎尼酸含量3.20%~3.75%。15批雪莲细胞培养物指纹图谱相似度大于0.9,特征图谱中呈现10个特征峰,指认峰2为绿原酸、峰4为紫丁香苷、峰7为1,5-二咖啡酰奎尼酸。结论:本研究建立了雪莲细胞培养物的质量标准,建议醇溶性浸出物不得低于29.4%;雪莲细胞培养物中的总黄酮类成分含量不得低于10.0%,绿原酸、紫丁香苷、1,5-二咖啡酰奎尼酸的含量应分别不得低于0.86%、0.34%、3.20%,为天山雪莲药制品的工业化生产提供了参考。Objective:To establish the quality standard of Saussurea involucrata cell cultures.Methods:According to the appendix of Chinese Pharmacopoeia(2020 edition),the general examination items and qualitative and quantitative indexes of S.involucrata cell cultures were established.The content of total flavonoids in Saussurea involucrata cell culture was determined by ultraviolet spectrophotometry with rutin as the control substance;the content of chlorogenic acid,syringin and 1,5-dicaffeoylquinic acid was determined by HPLC simultaneously,and the characteristic chromatogram was established.The similarity of characteristic chromatogram was analyzed by the software Chinese Medicine Chromatographic Fingerprint Similarity Evaluation system(2012 edition).Results:The total flavonoid content of 15 batches of S.involucrata cell cultures was between 10.0%and 11.0%;the content of chlorogenic acid was between 0.86%and 1.09%;the content of syringin was between 0.34%and 0.48%;1,5-The content of dicaffeoylquinic acid was between 3.20%and 3.75%.The similarity of fingerprint patterns of 15 batches of S.involucrata cell cultures was greater than 0.9,and there were 10 characteristic peaks in the characteristic pattern.Peak 2 was chlorogenic acid,peak 4 was syringin,and peak 7 was 1,5-dicaffeoylquinic acid.Conclusion:In this study,the quality standard of S.involucrata cell cultures is established,and it is suggested that the alcohol soluble extract should not be less than 29.4%;the content of total flavonoids in S.involucrata cell cultures should not be less than 10.0%,and the content of chlorogenic acid,syringin and 1,5-dicaffeoylquinic acid should not be less than 0.86%,0.34%and 3.20%,respectively,which provides a reference for the industrial production of S.involucrata medicinal products.

关 键 词：雪莲细胞培养物 定性定量 总黄酮 绿原酸 紫丁香苷 1 5-二咖啡酰奎尼酸 特征图谱 质量标准 

分 类 号：R284.1[医药卫生—中药学]