广西柑橘碎叶病和叶斑病调查及其病原的遗传多样性分析  

Investigation and the Genetic Diversity Analysis of the Pathogenic Virus Causing Citrus Tatter Leaf Disease and Citrus Leaf Spot Disease in Guangxi

作　　者：张金珠 邹承武 黄俊源[2] 邓崇岭 陈保善 张木清 ZHANG Jinzhu;ZOU Chengwu;HUANG Junyuan;DENG Chongling;CHEN Baoshan;ZHANG Muqing(State Key Laboratory for Conservation and Utilization of Sub-tropical Agro-Bioresources,Nanning,Guangxi 530005,China;College of Agricultural,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Academy of Specialty Crops,Guilin,Guangxi 541004,China)

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室,广西南宁530005 [2]广西大学农学院,广西南宁530004 [3]广西特色作物研究院,广西桂林541004

出　　处：《热带作物学报》2021年第5期1414-1423,共10页Chinese Journal of Tropical Crops

基　　金：广西科技创新发展专项(No.AA18118046)。

摘　　要：调查了广西南宁、崇左、柳州、桂林、玉林、河池、贺州及防城港等主要柑橘种植区的柑橘碎叶病和叶斑病的发生情况,并对其病原柑橘碎叶病毒(Citrus tatter leaf virus,CTLV)、苹果茎沟病毒(Apple stem grooving virus,ASGV)和柑橘叶斑病毒(Citrus leaf blotch virus,CLBV)进行了RT-PCR检测、病毒序列测定和遗传多样性分析。结果发现,在所有疑似柑橘病毒病样品中,CTLV检出率为7.3%,CLBV检出率为5.5%,ASGV检出率为3.0%。其中在南宁、桂林和玉林3个主要柑橘种植区采集的疑似柑橘病毒病样品的3种病毒病检出率合计分别为21.2%、28.6%和7.6%,且存在多种病毒复合侵染的现象。将克隆到的上述3个病毒分离物的病毒片段,分别与已报道的相应病毒的代表性分离物的病毒片段进行系统进化分析,结果表明,ASGV和CTLV的中国分离物在进化关系上均呈寄主相关性,ASGV的日本分离物则与地理位置相关性更强,而韩国和印度的分离物未表现出明显的寄主相关性和地理位置相关性。柑橘和苹果的ASGV和CTLV分离物均表现与地理位置相关,而梨的ASGV和CTLV分离物却与地理位置和寄主均不相关;CLBV分离物则呈明显的寄主相关性。本研究首次报道了广西柑橘碎叶病和柑橘叶斑病的发生情况及其病原的遗传多样性与系统进化关系,为柑橘病毒病的诊断和防治提供参考依据。The occurrence of citrus tatter leaf disease and citrus leaf spot disease were investigated in the main planting areas of citrus in Nanning,Chongzuo,Liuzhou,Guilin,Yulin,Hechi,Hezhou,and Fangchenggang of Guangxi.RT-PCR detection,viral genome sequencing and genetic diversity analysis were carried out for the pathogenic viruses,including Citrus tatter leaf virus(CTLV),Apple stem grooving virus(ASGV),and Citrus leaf blotch virus(CLBV).The results showed that the detection rates of CTLV,ASGV,and CLBV in suspected viral samples collected from the citrus-producing areas in Nanning,Guilin,and Yulin was 21.2%,28.6%,and 7.6%,respectively.Additionally,the mixed infection of multiple viruses was detected.Among the samples tested,the detection rate of CTLV was the highest(7.3%),followed by CLBV(5.5%),and ASGV(3.0%).The genetic diversity analysis and phylogenetic analysis were carried out by comparing the sequences of the three virus isolates cloned in this study with those of several representative viruses reported.The results demonstrated that both ASGV and CTLV isolates in China presented host correlation in the evolutionary relationship,while ASGV isolates in Japan showed a stronger association with geographical location.Still,the strains in South Korea and India did not show significant host or geographical location correlation.The isolates of ASGV and CTLV on citrus and apple presented a relationship with geographic location,while their isolates on pear showed no association with geographic location or host.Moreover,all strains of CLBV showed a distinct relationship with the host.It is the first time to report the occurrence of citrus tatter leaf disease and citrus leaf spot disease in Guangxi and the genetic diversity of their pathogenic viruses,which would provide a reference for the diagnosis and control of citrus virus diseases.

关 键 词：柑橘 病毒病调查 病毒检测 遗传多样性分析 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治]