外周血非编码微小RNA与HIV-1感染者病情进展的关系研究  

Study on relationship between non-coding microRNA in peripheral blood and disease progression of HIV-1 infectors

作　　者：程跃武[1] 张会平 谢天胜 CHENG Yue-wu;ZHANG Hui-ping;XIE Tian-sheng(The Center for Disease Control and Prevention of Shangcai County,Shangcai,Henan 463800,China;不详)

机构地区：[1]河南省上蔡县疾病预防控制中心,河南驻马店463800 [2]浙江科技学院生物与化学工程学院,杭州310023 [3]浙江中德生命健康教育研究院,杭州310023

出　　处：《疾病预防控制通报》2021年第2期14-17,共4页Bulletin of Disease Control & Prevention(China)

基　　金：浙江省卫生厅课题(2014KYA243)。

摘　　要：目的检测HIV-1感染者外周血微小RNA-155(miR-155)水平,并研究其与疾病进展的关系。方法收集121例接受抗病毒治疗(HAART)HIV-1感染者和43例未接受HAART的感染者,实时荧光定量PCR法测定miR-155表达水平,流式细胞术检测CD3^(+)CD4^(+)和CD3^(+)CD8^(+)T细胞数及其活化细胞的比例(CD4^(+)CD38^(+)%和CD8^(+)CD38^(+)%)。结果HIV-1感染者特别是治疗无效组外周血单核细胞(PBMC),CD4^(+)和CD8^(+)T细胞中的miR-155水平显著升高(F=11.317,P<0.01)。治疗无效者和未HAART治疗者CD38^(+)的CD4^(+)和CD8^(+)T细胞比例高于正常对照和治疗有效组(F=5.813,P<0.05),miR-155表达水平与T细胞免疫活化正相关(r=0.581,P=0.037;r=0.732,P=0.001)。结论本研究提示HIV-1感染者外周血miR-155表达水平显著升高,并与T细胞免疫活化正相关,可以作为HIV-1免疫应答的指标之一。Objective To detect the level of microRNA-155(miR-155) in the peripheral blood of HIV-1 infectors, and research its relationship to the disease progression. Methods Totally 121 HIV-1 cases treated by HAART and 43 cases not treated by HAART were selected for detecting the expressing level of miR-155 with real-time fluorescent quantitative PCR method, number of CD3^(+)CD4^(+), CD3^(+)CD8^(+)T cells and the proportion of their active cell(CD4^(+)CD38^(+)%, CD8^(+)CD38^(+)%) were detected with flow cytometry method. Results The level of miR-155 in peripheral blood mononuclear cell(PBMC), CD4^(+)and CD8^(+)T cells significantly increased(F=11.317, P<0.01) in the HIV-1 infectors, especially in the ineffective treatment group. The proportion of CD4^(+)and CD8^(+)T cell of CD38^(+)were significantly higher(F=5.813, P<0.05) in the cases with ineffective treatment and without HAART treatment than in normal control and effective treatment group. The expressing level of MiR-155 had positive correlation with immune activation of T cells(r=0.581, P=0.037;r=0.732, P=0.001).Conclusions The expressing level of miR-155 obviously increases in the peripheral blood of HIV-1 cases, and shows positive correlation with immune activation of T cells in this study. It can be one of the indicators of the immune response of HIV-1.

关 键 词：HIV-1 HAART MIR-155 CD38 免疫应答 

分 类 号：R512.91[医药卫生—内科学]