貂源沙门菌血清型调查及其对小鼠的致病性  

Survey on serotypes of Salmonella isolated from mink and its pathogenesis in mice

作　　者：周国栋 朱倩 时文健 袁朋[1] 姜世金[1] 谢之景[1] ZHOU Guodong;ZHU Qian;SHI Wenjian;YUAN Peng;JIANG Shijin;XIE Zhijing(College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian 271018,China)

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《兽类学报》2021年第3期245-253,共9页Acta Theriologica Sinica

基　　金：山东省现代农业产业技术体系特种经济动物创新团队项目(SDAIT-21-07)。

摘　　要：采用选择性培养、生化鉴定、血清型鉴定等方法,对从53份水貂病料中分离的35株沙门菌(Salmonella)进行了鉴定,分离率66.04%(35/53),其中34株为肠炎沙门菌(Salmonella enteritidis,Se),分别命名为Se-SD-1~Se-SD-34,1株为鼠伤寒沙门菌(S.typhimurium,St),命名为St-SD-1,这表明沙门菌在山东省诸城市水貂群中流行。采用PCR方法对35株沙门菌的10个毒力基因检测,结果invA和mgtC的携带率为100%(35/35),sopE和tolC 97.14%(34/35),stn 94.29%(33/35),avrA 88.57%(31/35),spiA 82.86%(29/35),hilC 68.57%(24/35),spvB 62.86%(22/35)和pefA 42.86%(15/35),毒力基因在不同菌株的分布不同。对阿莫西林等11种药物敏感性试验结果表明,35株水貂沙门菌存在不同程度的耐药,34株Se普遍耐药,其中对新霉素的耐药率最高(15/34),并存在对多种药物耐药现象,1株St耐药不严重,仅对四环素耐药。基于血清型、毒力基因检测结果,选取了Se-SD-1和St-SD-1作为代表性菌株,以小鼠为实验动物,进行了致病性研究。结果表明,Se-SD-1和St-SD-1均可引起小鼠发病,死亡率与接种剂量相关。该研究丰富了水貂沙门菌病的研究数据。有必要进一步开展水貂沙门菌的病原学和流行病学监测,具有重要的公共卫生学意义。Thirty-five(66.04%) Salmonella strains were isolated from 53 farmed mink samples collected in Zhucheng, Shandong Province, China, which were identified using selective culture, biochemical assays, and serotyping assays.Thirty-four(97.14%) of the 35 isolates were identified as Salmonella enteritidis(Se),named as Se-SD-1-Se-SD-34,followed by S.typhimurium(St)(1/35),named as St-SD-1.The 10 virulence-associated genes of Salmonella were tested using PCR.The prevalence of invA and mgtC genes for the isolates were 100%(35/35),sopE and tolC genes 97.14%(34/35),stn gene 94.29%(33/35),avrA gene 88.57%(31/35),spiA gene 82.86%(29/35),hilC gene 68.57%(24/35),spvB gene 62.86%(22/35) and pefA genes 42.86%(15/35).It demonstrated that the virulence gene contents were different among the Salmonella isolates.Antimicrobial susceptibility of 11 antimicrobials(amoxicillin etc.) for the isolates was performed using disk diffusion method.Thirty-four Se isolates exhibited multidrug resistance and cross resistance, and the highest level of resistance was found to kanamycin(15/34).The St isolate was only resistant to tetracycline.According to serotypes and virulence gene contents of the isolates, Se-SD-1 and St-SD-1 were selected for mouse experiments.As a result, Se-SD-1 and St-SD-1 caused the diseases in mice, and the inoculation doses could have influences on mouse mortality.This study enriches the research data of mink salmonellosis.It is necessary to further carry out the etiology and epidemiological surveillance of salmonella in mink for public health.

关 键 词：水貂 肠炎沙门菌 鼠伤寒沙门菌 耐药性 致病性 

分 类 号：Q955[生物学—动物学]