基质细胞衍生因子-1α联合自体富血小板血浆修复小鼠皮肤损伤实验研究  

Experimental study of stromal cell derived factor-1α combined with autologous platelet-rich plasma to repair skin injury in mice

作　　者：凌云浩 许丽玉 郭毅斌[1] 刘小飞 郑健生[1] Ling Yunhao;Xu Liyu;Guo Yibin;Liu Xiaofei;Zheng Jiansheng(Department of Burns and Plastic Surgery,the 909th Hospital of Chinese People′s Liberation Army,Southeast Affiliated Hospital of Xiamen University,Zhangzhou 363000,China)

机构地区：[1]联勤保障部队第九〇九医院厦门大学附属东南医院烧伤整形科,漳州363000

出　　处：《中华损伤与修复杂志（电子版）》2021年第3期239-244,共6页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：福建省自然科学基金(2019J01143)。

摘　　要：目的 探讨基质细胞衍生因子-1α(SDF-1α)联合自体富血小板血浆(PRP)在小鼠皮肤损伤修复过程中的作用效果,并分析其作用机制。 方法 选取6~8周龄健康C57小鼠24只,对24只小鼠分别抽取颈动脉血制备PRP;分别在每只小鼠脊柱2侧对称部位作直径为1.0 cm的圆形全层皮肤损伤创面,共建立48个创面,小鼠按随机数字表法分为观察组和对照组;每组各12只小鼠24个创面。48个创面均予PRP覆盖,观察组经皮下创面内予SDF-1α溶液(100μg SDF-1α+100μL 0.9%氯化钠溶液)从正常皮肤处经皮下注射至创面内,对照组同时采取同样方法注射200μL 0.9%氯化钠溶液,均连续注射7 d,2次/d,12 h/次。采用酶联免疫吸附试验(ELISA)检测对比造模后第7、14、21天2组创面血小板内皮细胞生长因子(VEGF)和转化生长因子β1(TGF-β1)表达量,采用蛋白质免疫印迹法对比造模后第7、14天2组创面VEGF蛋白和Samd1蛋白表达量,对比2组造模后第7、14、21天创面收缩情况及创面愈合时间。数据进行 t 检验。 结果 造模后第7、14天,观察组组织中VEGF含量分别为(0.66±0.07)、(0.49±0.07)pg/mL,均高于对照组[(0.45±0.05)、(0.45±0.06)pg/mL],差异均有统计学意义 (t=10.83、2.25,P <0.05);造模后第21天,观察组组织中VEGF含量[(0.44±0.06)pg/mL]和对照组[(0.43±0.06)pg/mL]相比,差异无统计学意义(t=0.30,P >0.05)。造模后第7、14天,观察组组织中TGF-β1含量分别为(0.54±0.05)、(0.38±0.04)pg/mL,均高于对照组[(0.34±0.04、0.35±0.05)pg/mL],差异均有统计学意 义(t=13.76、2.52,P< 0.05);造模后第21天,观察组组织中TGF-β1含量[(0.33±0.04)pg/mL]和对照组[(0.35±0.04)pg/mL]相比,差异无统计学意义 (t=-1.66,P> 0.05)。造模后第7、14天,观察组VEGF蛋白均高于对照组,差异有统计学意义 (t=7.31、4.29,P <0.05),观察组Samd1蛋白均高于对照组,差异有统计学意义 (t=2.74、11.13,P <0.05)。造模后第7、14天,观察组的创面愈合收缩�To explore the effects of stromal cell derived factor-1α(SDF-1α)combined with platelet-rich plasma(PRP)in mouse skin injury repair and to explore its mechanism.MethodsTwenty-four healthy C57 mice aged 6 to 8 weeks were selected and carotid blood was extracted from each mouse to prepare PRP gel.A total of 48 round full-thickness skin injury wounds with a diameter of 1.0 cm were made on 2 symmetrical parts of the spine of each mouse.The mice were divided into observation group and control group according to random number table method.There were 24 wounds,12 mice in each group.All 48 wounds were covered with PRP gel.In the observation group,SDF-1αsolution(100μg SDF-1α+100μL 0.9%sodium chloride solution)was injected subcutaneously into the wound from the normal skin.The control group was injected with 200μL 0.9%sodium chloride solution.All the 48 wounds were injected continuously for 7 d,twice a day,12 h a time.The expression levels of platelet endothelial cell growth factor(VEGF)and transforming growth factor(TGF-β1)in the wound of the two groups were measured and compared by enzyme-linked immunoadsordent assay(ELISA)at 7,14 and 21 days after modeling.Western blotting was used to compare the expression levels of VEGF protein and SAMD1 protein in the two groups at 7,14 and 21 days after modeling.Wound healing shrinkage rate at 7,14 and 21 days after modeling and wound healing time were compared between the two groups.Data was processed withttest.ResultsAt 7,14 days after modeling,the VEGF content in the observation group was(0.66±0.07,0.49±0.07)pg/mL,which were higher than those in the control group[(0.45±0.05),(0.45±0.06)pg/mL],and the differences were statistically significant(t=10.83,2.25;P<0.05).At 21 days after modeling,there was no significant difference in VEGF content between the observation group[(0.44±0.06)pg/mL]and the control group[(0.43±0.06)pg/mL](t=0.30,P>0.05).At 7,14 days after modeling,the content of TGF-β1 in the observation group was(0.54±0.05),(0.38±0.04)pg/mL,which were greater

关 键 词：小鼠 创伤和损伤 富血小板血浆 伤口愈合 基质细胞衍生因子-1Α 

分 类 号：R641[医药卫生—外科学]