杜仲多糖通过TGF-β1/Smad信号通路抑制ox-LDL诱导的血管平滑肌细胞增殖和迁移  

Eucommia ulmoides polysaccharide inhibits the proliferation and migration of vascular smooth muscle cells induced by oxidized low density lipoprotein through TGF-β1/Smad signaling pathway

作　　者：邓华[1] DENG Hua(Department of Internal Medicine,Department of Clinical Medicine,Sichuan College of Traditional Chinese Medicine,Mianyang,Sichuan 621000,China)

机构地区：[1]四川中医药高等专科学校临床医学系内科教研室,四川省绵阳市621000

出　　处：《中国动脉硬化杂志》2021年第6期499-505,共7页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨杜仲多糖对氧化型低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞(VSMC)增殖及迁移的影响。方法体外培养大鼠VSMC,用ox-LDL及不同浓度的杜仲多糖处理。甲基噻唑基四唑法检测细胞增殖;酶联免疫吸附试验检测白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的水平;Transwell实验检测细胞迁移;划痕实验检测划痕愈合率;Western blot检测转化生长因子β1(TGF-β1)/Smad信号通路相关蛋白Smad2/3、p-Smad2/3的表达。结果ox-LDL处理VSMC后,细胞周期蛋白D1(Cyclin D1)、细胞周期蛋白依赖激酶2(CDK2)、p-Smad2/3蛋白水平显著升高(P<0.05),细胞培养上清液中IL-6、TNF-α水平显著升高(P<0.05),细胞活力显著升高(P<0.05),迁移细胞数、划痕愈合率显著增高(P<0.05);杜仲多糖处理后可明显逆转ox-LDL对VSMC增殖及迁移的作用(P<0.05);添加TGF-β1后,Cyclin D1、CDK2、p-Smad2/3蛋白水平显著升高(P<0.05),IL-6、TNF-α水平显著升高(P<0.05),细胞活力显著升高(P<0.05),迁移细胞数显著增多(P<0.05)。结论杜仲多糖可能通过抑制TGF-β1/Smad信号通路的活化从而抑制ox-LDL诱导的VSMC增殖及迁移。Aim To investigate the effect of eucommia ulmoides polysaccharide on the proliferation and migration of vascular smooth muscle cells(VSMC)induced by oxidized low density lipoprotein(ox-LDL).Methods Rat VSMCs were cultured in vitro and treated with ox-LDL and different concentrations of eucommia ulmoides polysaccharide.Methyl thiazolyl tetrazolium assay was used to detect cell proliferation.The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay.Transwell experiment was used to detect cell migration.The scratch healing rate was detected by scratch test.Western blot was used to detect the expressions of transforming growth factor-β1(TGF-β1)/Smad signaling pathway-related proteins Smad2/3 and p-Smad2/3.Results After ox-LDL treatment,the levels of Cyclin D1,cyclin dedependent kinase 2(CDK2)and p-Smad2/3 proteins were significantly increased(P<0.05),the levels of IL-6 and TNF-αin cell culture supernatant were significantly increased(P<0.05),the cell viability was significantly increased(P<0.05),and migration cell number and scratch healing rate were significantly increased(P<0.05).The effect of ox-LDL on VSMC proliferation and migration was significantly reversed after treatment with eucommia ulmoides polysaccharide(P<0.05).After adding TGF-β1,the levels of Cyclin D1,CDK2 and p-Smad2/3 proteins were significantly increased(P<0.05),the levels of IL-6 and TNF-αwere significantly increased(P<0.05),the cell viability was significantly increased(P<0.05),and the number of migrating cells was significantly increased(P<0.05).Conclusion Eucommia ulmoides polysaccharide may inhibit the proliferation and migration of VSMC induced by ox-LDL by inhibiting the activation of TGF-β1/Smad signaling pathway.

关 键 词：杜仲多糖 TGF-Β1/SMAD信号通路 氧化型低密度脂蛋白 血管平滑肌细胞 细胞增殖 细胞迁移 

分 类 号：R96[医药卫生—药理学] R5[医药卫生—药学]