急性心肌梗死与冠状动脉血内皮细胞源微粒、组织因子和白细胞介素-33的相关性研究  

Correlations between acute myocardial infarction and endothelial microparticles,tissue factor,and interleukin-33 of coronary artery blood

作　　者：袁玉娟[1] 李吉斌 李昊[3] 穆叶赛·尼加提[3] YUAN Yujuan;LI Jibin;LI Hao;Muyesai·Nijiati(Department of Cardiology,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi,830001,China;Department of Ultrasound,People’s Hospital of Xinjiang Uygur AutonomousRegion;Emergency Center,People’s Hospital of Xinjiang Uygur Autonomous Region)

机构地区：[1]新疆维吾尔自治区人民医院心血管内科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院超声科 [3]新疆维吾尔自治区人民医院急救中心

出　　处：《临床心血管病杂志》2021年第4期328-332,共5页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:81760068);新疆维吾尔自治区人民医院院内项目(No:20190207)。

摘　　要：目的:分析急性心肌梗死(AMI)患者冠状动脉(冠脉)血内皮细胞源微粒(EMPs)、组织因子(TF)和白细胞介素-33(IL-33)水平,探讨EMPs、TF和IL-33与冠脉斑块局部急性血栓形成事件的相关性。方法:冠脉介入术中收集27例AMI患者(AMI组)和30例稳定型冠心病(SCAD)患者(SCAD组)冠脉血标本。采用流式细胞术对EMPs(CD31)进行定性和定量检测,采用ELISA法测定TF和IL-33蛋白水平。结果:AMI组EMPs水平高于SCAD组[11.10(8.27,13.20)%∶3.92(2.80,7.02)%,P<0.001],AMI组TF水平高于SCAD组[(303.80.12±42.04)pg/mL∶(197.12±38.05)pg/mL,P<0.001],AMI组IL-33水平高于SCAD组[(138.29±47.64)pg/mL∶(96.93±28.87)pg/mL,P<0.001]。根据ROC曲线分析EMPs、TF和IL-33诊断AMI的价值,其曲线下面积(AUC)分别为0.888、0.962和0.778。结论:AMI患者冠脉血EMPs、TF和IL-33蛋白水平升高,EMPs、TF蛋白和IL-33蛋白有可能参与急性冠脉血栓事件。Objective:The study investigated the level of endothelial microparticles(EMPs),tissue factor(TF)and interleukin(IL)-33 protein of coronary blood in patients with acute myocardial infarction(AMI),and explored the correlations between EMPs,TF and IL-33.Methods:The level of EMPs,IL-33 and TF protein of coronary blood in AMI and stable coronary artery disease(SCAD)patients were tested by flow cytometry and ELISA.Results:In the study,we demonstrated that the AMI group had a higher level of EMPs,TF,and IL-33 protein than the SCAD patients in coronary blood(P<0.001),11.10%(8.27%,13.20%)vs 3.92%(2.80%,7.02%)for EMPs,(303.80±42.04)pg/mL vs(197.12±38.05)pg/mL for TF protein,(138.29±47.64)pg/mL vs(96.93±28.87)pg/mL for IL-33 protein.According to the ROC curve analysis,the areas under the curve(AUC)EMPs,TF,and IL-33 protein were 0.888,0.962 and 0.778.Conclusion:The coronary blood of level of EMPs,TF,and IL-33 protein in AMI patients may be involved in the process of acute thrombosis and become a potential risk factor for cardiovascular disease.

关 键 词：急性心肌梗死 内皮细胞源微粒 组织因子蛋白 IL-33 

分 类 号：R541.4[医药卫生—心血管疾病]