荧光猝灭型硼氟二吡咯类Cu^(2+)探针的合成及其生物细胞成像的应用  

Fluorescent Turn‑Off Boron Fluoride Dipyrrole Probe for Cu^(2+):Synthesis and Application in Living Cells Imaging

作　　者：边永军 渠星宇 李芬芳[1] 张政委 李建晴[1] 陈勇强 沈珍 BIAN Yong‑Jun;QU Xing‑Yu;LI Fen‑Fang;ZHANG Zheng‑Wei;LI Jian‑Qing;CHEN Yong‑Qiang;SHEN Zhen(Department of Chemistry and Chemical Engineering,Jinzhong University,Jinzhong,Shanxi 030619,China;State Key Laboratory of Coordination Chemistry,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China)

机构地区：[1]晋中学院化学化工系,晋中030619 [2]南京大学化学化工学院,配位化学国家重点实验室,南京210023

出　　处：《无机化学学报》2021年第6期1089-1096,共8页Chinese Journal of Inorganic Chemistry

基　　金：山西省高等学校科技创新项目(No.2019L0894);晋中学院“1331工程”光电材料重点创新团队(No.jzxycxtd2017007);山西省‘1331工程’重点创新团队建设计划(No.PY201817);山西省高等学校教学改革创新项目(No.J2020317);晋中学院教学改革创新项目(No.Jg202039)资助。

摘　　要：设计合成了一个反应型的硼氟二吡咯(BODIPY)类荧光探针1,该探针以吡啶‑2‑羧酸苯酚酯基为识别基团。通过^(1)H NMR、^(13)C NMR和HRMS表征了1的结构,并解析了其晶体结构。光谱分析实验结果显示,探针1具有高的荧光量子产率(0.79),对Cu^(2+)具有较强的选择性识别性能,并能成功应用到生物细胞中Cu^(2+)的成像检测。A fluorescent probe 1,based on boron fluoride dipyrrole(BODIPY)chromophore containing phenyl picolinate as a recognition moiety,was designed,synthesized,and characterized by^(1)H NMR,^(13)C NMR,HRMS and Xray diffraction.Probe 1 had a high fluorescence quantum yield(0.79),and could selectively and sensitively recognize Cu^(2+)from other cations by a fluorescence quenching method.The limit of detection of probe 1 was 0.15μmol·L-1.In addition,the recognition mechanism of the fluorescent response to copper ions was verified by HRMS in this work.CCDC:2062769.

关 键 词：硼氟二吡咯亚甲基 铜离子 荧光探针 荧光猝灭 

分 类 号：O614.121[理学—无机化学]