基于16S rRNA测序研究葛根芩连汤对菌群失调性腹泻大鼠肠道菌群结构的影响  

Effect of Gegen Qinliantang on Structure of Intestinal Flora in Dysbacterial Diarrhea Rats Based on 16S rRNA Sequencing

作　　者：张庚鑫 杜海洋 王平 杨光勇 涂小华 田维毅 王文佳 何光志 ZHANG Geng-xin;DU Hai-yang;WANG Ping;YANG Guang-yong;TU Xiao-hua;TIAN Wei-yi;WANGWen-jia;HE Guang-zhi(School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,GuiyaNg 550025,China)

机构地区：[1]贵州中医药大学基础医学院,贵阳550025

出　　处：《中国实验方剂学杂志》2021年第11期19-26,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2018年度学术新苗项目(黔科合平台人才[2018]5766号-16);国家自然科学基金地区科学基金项目(82060796);贵州省科学技术基金项目(黔科合基础[2020]1Y392);贵州省教育厅创新群体重大研究项目(黔教合KY字[2016]036);贵州省科技创新人才团队建设专项(黔科合平台人才[2020]5010);贵州省发展和改革委员会工程研究中心建设项目[黔发改高技(2020)896号]。

摘　　要：目的:采用16S rRNA技术研究葛根芩连汤对菌群失调性腹泻大鼠肠道菌群结构的影响。方法:将60只健康SD大鼠随机分为6组,分别为正常组、模型组、葛根芩连汤高、中、低剂量组,丽珠肠乐组,每组10只。除正常组外,每日按头孢拉定178.6 mg·kg^(-1),硫酸庆大霉素31.25 mg·kg^(-1)混合抗生素进行灌胃造模,造模成功后,葛根芩连汤高、中、低剂量组灌胃葛根芩连汤(7.02,3.51,1.755 g·kg^(-1)),丽珠肠乐组按0.125 g·kg^(-1)灌胃,正常组与模型组给予无菌蒸馏水灌胃,体积分数均为10 mL·kg^(-1),连续给药7 d,将大鼠麻醉后取大鼠结肠内容物,提取粪便DNA后进行16S rRNA高通量测序并分析其结果。结果:葛根芩连汤明显调节菌群失调性腹泻模型大鼠的物种数量及Alpha与Beta多样性,提高菌群生物丰富度指数与多样性指数,正向调节菌群失调性腹泻模型大鼠的3种差异菌门(厚壁菌门、变形菌门、拟杆菌门)与14种差异菌属(拟杆菌属、狄氏副拟杆菌属、布劳特氏菌属等)。结论:葛根芩连汤对菌群失调性腹泻模型大鼠肠道菌群有调节作用,揭示了肠道菌群与菌群失调性腹泻之间的生理病理机制。Objective: To study the effect of Gegen Qinliantang(GQT)on the structure of intestinal flora in dysbacterial diarrhea rats by 16S rRNA sequencing. Method: Sixty healthy SD rats were randomly and equally divided into a control group,a model group,high-,medium-,and low-dose GQT groups,and a Bifidobiogen group. The rat model was induced in the five groups except the control group by administration of mixed antibiotics(178.6 mg·kg^(-1) cefradine and 31.25 mg·kg^(-1) gentamicin sulfate)according to the dose. Drug intervention was carried out in each group(7.02,3.51,and 1.755 g·kg^(-1) GQT for the high-,medium-,and lowdose GQT groups,0.125 g·kg^(-1) bifidobacterium capsules for the Bifidobiogen group,and sterile distilled water for the control and model groups)with a volume of 10 mL·kg^(-1) for seven days. Colon contents of rats were obtained under anesthesia. The extracted fecal DNA underwent 16S rRNA high-throughput sequencing and the results were analyzed. Result: GQT was proved capable of adjusting the species number and Alpha and Beta diversity,improving the biological richness and diversity of the flora,and positively regulating three differential phyla(Firmicutes, Proteobacteria, and Bacteroidetes) and 14 differential genera(Bacteroides,Parabacteroides,Blautia,etc.)in rat model of dysbacterial diarrhea. Conclusion: The present study confirmed the regulatory effect of GQT on intestinal flora of dysbacterial diarrhea rats,and revealed the physiological and pathological mechanism between intestinal flora and dysbacterial diarrhea.

关 键 词：16S rRNA 葛根芩连汤 肠道菌群 菌群结构 菌群失调性腹泻 

分 类 号：R2-0[医药卫生—中医学] R22