按压对大鼠肌筋膜激痛点软组织张力的影响及其作用机制研究  

The effect of pressing on the soft tissue tension of myofascia ltrigger points in rats and its mechanism

作　　者：蒋全睿 吴琼 匡小霞 危威 江玉婷 袁媛 李武[1] 李江山[1] JIANG Quanrui;WU Qiong;KUANG Xiaoxia;WEI Wei;JIANG Yuting;YUAN Yuan;LI Wu;LI Jiangshan(College of Acupuncture&Moxibustion and Tui-Na,Hunan University of Chinese Medicine,Changsha 410208,China;TCM Department,The Second Hospital,University of South China,Hengyang 421000,China)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208 [2]南华大学附属第二医院中医科,衡阳421000

出　　处：《中国疼痛医学杂志》2021年第5期335-341,共7页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金面上项目(81973975);湖南中医药大学开放性基金项目(2018ZYX07)。

摘　　要：目的:探讨按压对大鼠肌筋膜激痛点(myofascial trigger point,MTrP)软组织张力的影响及其作用机制。方法:雄性SD大鼠48只,随机分为4组:空白组、模型组、利多卡因组和按压组,每组12只。模型组、利多卡因组和按压组采用离心运动结合钝性打击的方法建立MTrP大鼠模型。造模评价后,利多卡因组给予激痛点局部注射利多卡因干预,按压组给予激痛点局部按压干预。采用软组织张力测定仪检测大鼠激痛点软组织张力,之后在激痛点局部取材。用免疫组化法检测P物质(substance P)和降钙素基因相关肽(calcitonin gene-related peptide,CGRP)表达。用试剂盒以比色法检测游离Ca^(2+)含量。用Western Blot法检测二氢吡啶受体α1(dihydropyridine receptorα1,DHPRα1)、利若丁受体(ryanodine receptor,RyR)和乙酰胆碱酯酶(acetyl cholinesterase,AChE)表达。结果:(1)与空白组相比,模型组激痛点软组织张力、Ca^(2+)、P物质和CGRP含量增高(P<0.05),DHPRα1、RyR和AChE含量降低(P<0.05);(2)与模型组相比,按压组和利多卡因组激痛点软组织张力、Ca^(2+)、P物质和CGRP含量降低(P<0.05),DHPRα1、RyR和AChE含量增高(P<0.05);(3)与利多卡因组相比,按压组软组织张力、DHPRα1、RyR、AChE、Ca^(2+)、P物质和CGRP含量差异均无统计学意义(P>0.05)。结论:按压可以有效降低激痛点软组织张力,其机制与促进DHPRα1、RyR和AChE表达,降低Ca^(2+)、P物质和CGRP含量有关。Objective:To investigate the effect and mechanism of pressing on soft tissue tension of myofascial trigger point(MTrP)in rats.Methods:Forty-eight male SD rats were randomly divided into four groups:blank group,model group,lidocaine group and pressing group,with 12 rats in each group respectively.In the model group,lidocaine group and pressing group,the MTrP model was established by eccentric exercising and blunt striking.After modeling evaluation,lidocaine group was given local injection of lidocaine at MTrP,and pressing group was given local pressing at MTrP.The soft tissue tension was measured by soft tissue tensiometer.Then local sample of MTrP was drawn.The substance P and calcitonin gene-related peptide(CGRP)were determined by immunohistochemistry.The contents of Ca^(2+)was detected by colorimetric kit.The dihydropyridine receptorα1(DHPRα1),ryanodine receptor(RyR)and acetyl cholinesterase(AChE)were detected by Western blot.Results:(1)Compared with the blank group,the soft tissue tension and the contents of substance P and CGRP in model group were increased(P<0.05),while the contents of DHPRα1,RyR and AChE were decreased(P<0.05)(2)Compared with the model group,the soft tissue tension and the contents of Ca^(2+),substance P and CGRP in pressing group and lidocaine group were decreased(P<0.05),while the contents of DHPRα1,RyR and AChE were increased(P<0.05).(3)Compared with lidocaine group,there were no significant differences of soft tissue tension,DHPRα1,RyR,AChE,Ca^(2+),substance P and CGRP in pressing group(P>0.05).Conclusion:The pressing can effectively reduce the soft tissue tension of MTrP.Its mechanism may be related to promoting the expression of DHPRα1,RyR and AChE,and reducing the contents of Ca^(2+),substance P and CGRP.

关 键 词：按压 激痛点 大鼠 软组织张力 DHPRα1 RYR 乙酰胆碱酯酶 机制研究 

分 类 号：R402[医药卫生—临床医学]