传统中药东亚钳蝎基因在毒液腺特征分布的转录组学分析  

Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands

作　　者：张鸿飞 葛得龙 黄晓斐 梅迪 郭莉 王艺蓉 李海梅 孙同毅[1] 贾光韬 李文辉[1,2] ZHANG Hong-fei;GE De-long;HUANG Xiao-fei;MEI Di;GUO Li;WANG Yi-rong;LI Hai-mei;SUN Tong-yi;JIA Guang-tao;LI Wen-hui(School of Biological Science and Technology,Weifang Medical University,Weifang Shandong 261053;Kunming Institute of Zoology,Kunming Yunnan 650223,China)

机构地区：[1]潍坊医学院山东省蛋白质与多肽药物工程实验室,山东潍坊261053 [2]中国科学院昆明动物研究所,云南昆明650223

出　　处：《昆明医科大学学报》2021年第5期35-40,共6页Journal of Kunming Medical University

基　　金：云南省科技厅重点领域科技计划基金资助项目(2019ZF003-002)。

摘　　要：目的研究东亚钳蝎Buthus martensii Karsch转录组信息特征。方法以山东省临沂地区特色东亚钳蝎为研究对象,利用二代高通量测序平台Illumina HiSeq 4000150PE分别对东亚钳蝎的前腹部、后腹部、毒液腺进行转录组测序。利用测定的转录组数据将毒液腺基因分别与前腹部和后腹部基因集进行比较,然后取两两比较之后的交集进行分析,筛选差异基因集进行GO和KO分析。结果GO功能分类共有60个大项,含有23个生物学途径,13个细胞组分和24个分子功能。富集最显著的前20个KEGG通路中与毒素密切相关的为钙离子信号通路、磷脂酶信号通路。结论通过无参转录组测序,构建了东亚钳蝎转录组序列数据库,为今后东亚钳蝎功能基因的挖掘提供了序列基础并为代谢产物的生物合成提供了数据支撑,为传统中药在临床应用乃至精准医学方面提供了新的思路。Objective To obtain the transcriptome dataset of Buthus martensii.Methods Taking the characteristic Buthus martensii in Linyi area of Shandong Province as the research object,the second-generation high-throughput sequencing platform Illumina HiSeq 4000150PE was used to sequence the transcriptome of the anterior abdomen,posterior abdomen and venom gland of Buthus martensii.The venom gland genes were compared with the preabdomen and the postabdomen gene sets,and then the intersection of the two comparison was analyzed.The screening differential gene sets were analyzed by GO and KO.Results There were 60 major categories of GO functional classification,containing 23 biological pathways,13 cellular components,and 24 molecular functions.Among the first 20 KEGG pathways that were most significantly enriched,the calcium ion signaling pathway and phospholipase signaling pathway were closely related to toxins.Conclusion In this study,we have constructed the transcriptome sequence database of B.martensii by nonparticipating transcriptome sequencing,which provids the sequence basis for the future research on the gene mining function of Buthus martensii and the research basis for the biosynthesis of metabolites.

关 键 词：东亚钳蝎 毒液腺 前腹部 后腹部 转录组测序 

分 类 号：R282.7[医药卫生—中药学]