恶性梗阻性黄疸患者与健康人粪菌群的对比  

A Comparative Study of Fecal Bacteria between Patients with Malignant Obstructive Jaundice and Healthy People

作　　者：孙岩波[1,2] 李为明 李树民[1,2] 林岳瀛 岑云云[1,2] 徐鹏远 SUN Yan-bo;LI Wei-ming;LI Shu-min;LIN Yue-ying;CEN Yun-yun;XU Peng-yuan(Dept.of Gastrointestinal Surgery,The 2nd Affiliated Hospital of Kunming Medical University.Kunming Yunnan 650101;Research Center for Surgical Clinical Nutrition of Yunnan Province,Kunming Yunnan 650101,China)

机构地区：[1]昆明医科大学第二附属医院胃肠外科,云南昆明650101 [2]云南省外科临床营养研究中心,云南昆明650101

出　　处：《昆明医科大学学报》2021年第5期87-91,共5页Journal of Kunming Medical University

基　　金：云南省外科临床营养研究中心基金资助项目(2018NS0317,2018NS0318);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2019FE001(-223)];云南省卫生健康委员会医学后备人才培养计划基金资助项目(H-2018067)。

摘　　要：目的初步探索恶性梗阻性黄疸患者粪菌群变化特征。方法以2019年1月至2019年5月入住昆明医科大学第二附属医院的87例恶行梗阻性黄疸患者为病例库,经纳入排除标准筛选,最终选取10例患者为研究对象、10例患者健康亲属作为对照组标本采集对象,记录年龄、性别、BMI;送检粪便测粪便中菌群16srDNA拷贝数。结果(1)实验组粪菌群丰度和多样性指数比对照组低,但无统计学意义(P>0.05);但是将丰度数据以群落Heatmap图呈现,直观差异明显;(2)将两组数据以菌属为单位按比例细分,发现实验组粪菌属组成较对照组发生了根本性的变化(P<0.05),其中大肠杆菌志贺菌、链球菌、韦荣氏球菌属、肠球菌属、拟杆菌属显著增加,而魏斯式菌属、梭状芽胞杆菌属等菌属大幅下降。结论恶性梗阻性黄疸患者患者粪菌群结构与正常对照组的粪便菌群结构之间存在明显差异,优势菌群菌属发生了根本性的改变。Objective To explore the changing characteristics of fecal bacteria in patients with malignant obstructive jaundice.Methods From January 2019 to May 2019,87 cases with malignant obstructive jaundice were taken as case bank and screened by inclusion and exclusion criteria.After that,10 patients and 10 healthy relatives of patients were selected,age,sex and BMI were recorded,and faeces were sent for 16srDNA copy number.Results(1)The fecal bacteria abundance and diversity index of the experimental group were lower than that of the control group,but there was no statistical significance(P<0.05).However,when the data of fecal bacteria abundance were presented in the Heatmap,the intuitive difference was more obvious.(2)The data of the two groups were subdivided by bacteria genus,and it was found that the composition of fecal bacteria genus in the experimental group changed fundamentally compared with that in the control group(P<0.05).Among them,Escherichia-Shigella,Streptococcus,Veillonella,Enterococcus,Akkermansia increased significantly,while the bacteria genera of wiesia and clostridium declined significantly.Whether the intestinal flora can be rebalanced may affect the therapeutic effect.Conclusion The fecal flora structure of patients with malignant obstructive jaundice is significantly different from that of normal control group,and the dominant flora genus has been fundamentally changed.

关 键 词：恶性梗阻性黄疸 粪菌群 平衡 

分 类 号：R459.3[医药卫生—治疗学]