无病毒百合‘Sorbonne’试管内小鳞茎直接再生体系建立  

Establishment of in vitro Direct Regeneration System on Virus-free Bulblets of‘Sorbonne’

作　　者：李雪艳[1] 胡新颖[1] 王伟东[1] 白一光[1] 杨迎东[1] LI Xueyan;HU Xinying;WANG Weidong;BAI Yiguang;YANG Yingdong(Institute of Flowers,Liaoning Academy of Agricultural Science,Shengyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院花卉研究所,辽宁沈阳110161

出　　处：《北方园艺》2021年第9期80-86,共7页Northern Horticulture

基　　金：辽宁省自然科学基金资助项目(20170540503,20170520065,2019-MS-193,2019-ZD-0379)。

摘　　要：以‘Sorbonne’种球为试材,采用RT-PCR技术检测获得无CMV、LSV、LMoV的‘Sorbonne’种球,剥取其中外层鳞片通过组培获得无菌试管苗,以无菌苗鳞片为次级外植体,研究了不同植物生长调节剂对试管内小鳞茎直接再生的影响,以期为繁育优质的无病毒百合种球提供参考依据。结果表明,试管内小鳞茎直接再生的最适培养基为MS+2,4-D 1.0mg·L^(-1),诱导率与诱导系数为100%、3.69;小鳞茎膨大最适培养基为MS+蔗糖90g·L^(-1);生根最适培养基为MS+NAA 0.1mg·L^(-1),移栽成活率可达100%。Taking‘Sorbonne’bulbs as test material,the‘Sorbonne’bulbs without CMV,LSV and LMoV virus were obtained by RT-PCR.Scales excised of in vitro stock were used as secondary explants to explore the effects of different plant growth regulators on the direct regeneration of bulblets in vitro,in order to provide reference for breading high-quality virusfree lily bulbs.The results showed that the optimal medium for direct regeneration of bulblets in vitro was MS+2,4-D 1.0mg·L^(-1),and the induction rate and induction coefficient were 100%and 3.69,respectively.The optimal medium for small bulb enlargement was MS+sucrose 90g·L^(-1),MS+NAA 0.1mg·L^(-1) was best for rooting,and the regenerated plantlets survived of‘Sorbonne’with highest rate of 100.00%.

关 键 词：百合 无病毒 试管小鳞茎 直接再生 原原种 

分 类 号：S644.1[农业科学—蔬菜学]