肛愈散对大鼠痔术后创面愈合、血清炎症因子及胶原蛋白表达影响的实验研究  

Effect of Gangyusan on wound healing, serum inflammatory factors and collagen expression after hemorrhoid operation in rats

作　　者：林巧娟 林晖[2] LIN Qiaojuan;LIN Hui(Graduate school,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Anorectal Department,Shanghai Changning Tianshan Traditional Chinese Medicine Hospital,Tianshan Branch of Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200051,China)

机构地区：[1]上海中医药大学研究生院,上海201203 [2]上海市长宁区天山中医医院,上海中医药大学附属龙华医院天山分院肛肠科,上海200051

出　　处：《上海中医药杂志》2021年第4期66-72,共7页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委资助项目(16401971500);上海市长宁区名医工作室(林晖)资助项目;上海市长宁区医学名专科(肛肠科)资助项目。

摘　　要：目的研究肛愈散对大鼠痔术后创面愈合中血清炎症因子TNF-α、IL-6、创面再生胶原蛋白表达水平及创面组织形态学的影响。方法取60只SPF级SD大鼠,随机分成空白对照组、模型对照组、痔疾洗液组及肛愈散低、中、高剂量组,每组10只。除空白对照组外,其余组均以75%冰醋酸注射肛周建立痔术后模型。造模成功后,空白对照组及模型对照组给予高压灭菌水熏洗,痔疾洗液组及肛愈散低、中、高剂量组给予相应药物熏洗治疗。治疗后取特定时间点(第1、3、7、11天)测量创面面积,计算愈合率,肉眼观察创面色泽、渗出;通过光镜观察炎症细胞浸润、成纤维细胞、肉芽组织增生等炎症反应及组织愈合情况。连续治疗10 d后,比较各组创面肉芽组织的组织病理,同时采用酶联免疫吸附(ELISA)法和荧光定量(qRT-RCR)检测大鼠血清中IL-6、TNF-α含量和创面组织中IL-6、TNF-αmRNA表达,采用Western blot法和免疫组化法检测大鼠创面组织中COL1、COL3蛋白表达水平。结果与模型对照组比较,肛愈散可明显降低大鼠创面组织IL-6、TNF-α mRNA表达及血清中炎症因子IL-6、TNF-α的含量(P<0.05);创面组织中COL1、COL3蛋白表达均明显增加(P<0.05)。光镜下肛愈散各治疗组可见新鲜肉芽组织生成,成纤维细胞排列整齐且新生毛细血管丰富,均优于模型对照组。结论肛愈散可降低大鼠血清和创面组织中IL-6、TNF-α等炎症因子的表达,促进创面肉芽组织、新生毛细血管、成纤维细胞生成,促进大鼠痔术后创面愈合。Objective To explore the influence of Gangyusan on serum inflammatory factors TNF-α, IL-6, expression of wound regeneration collagen and wound histomorphology in rats after hemorrhoid operation. Methods Sixty SPF SD rats were randomly divided into blank control group, model control group, hemorrhoid lotion group, and low, medium and high dose groups of Gangyusan(n=10). Except for the blank control group, the rats in other groups were injected with 75% glacial acetic acid around the anus to establish hemorrhoid operation model. After successful modeling, the blank control group and the model control group were given fumigation with high pressure sterilization water. The hemorrhoid lotion group and the low, medium and high dose groups of Gangyusan were given corresponding drug fumigation and washing treatment. After treatment, the wound area was measured, the healing rate calculated, and the color and exudation of the wound observed with naked eyes at specific time points(1 st, 3 rd, 7 th and 11 th day).Inflammatory reactions such as inflammatory cell infiltration,fibroblasts,granulation tissue proliferation and tissue healing were observed under light microscope. After 10 days of continuous treatment,the histopathology of granulation tissue in each group was compared;the contents of IL-6 and TNF-α in serum and the expression of IL-6 and TNF-α mRNA in wound tissue were detected by enzyme linked immunosorbent assay( ELISA) and fluorescence quantitative assay. The expression levels of COL1 and COL3 in wound tissue were detected by Western blot method and immunohistochemical method. Results Compared with the model control group,Gangyusan significantly reduced the expression of IL-6 and TNF-α mRNA in wound tissue and the content of inflammatory factors IL-6 and TNF-α in serum,while the protein expression of COL1 and COL3 in wound tissue increased significantly( P < 0.05). Under light microscope,the formation of fresh granulation tissue,neat arrangement of fibroblasts and abundant new capillaries were seen in each

关 键 词：中药熏洗 肛愈散 痔术后 创面愈合 炎症因子 胶原蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]