酸枣仁提取物调控cAMP-PKA通路促进小鼠体长增长及大鼠慢波睡眠的机制研究  

Mechanism of Ziziphi Spinosae Semen extract promoting bone growth in mice and slow wave sleep of rats by regulating cAMP-PKA pathway

作　　者：谢艳 张云芳 宁桃丽 邹吉锋 李少侠 薛彩霞 王娜 XIE Yan;ZHANG Yunfang;NING Taoli;ZOU Jifeng;LI Shaoxia;XUE Caixia;WANG Na(Luoyang Orthopedic Thraumalological Hospital of Henan Province,Henan Provincal Orthopedic Hospital,Luoyang,Henan 471000,China)

机构地区：[1]河南省洛阳正骨医院,河南省骨科医院,河南洛阳471000

出　　处：《上海中医药杂志》2021年第5期77-83,共7页Shanghai Journal of Traditional Chinese Medicine

基　　金：河南省中医药科学研究专项(2017ZY2131)。

摘　　要：目的探讨酸枣仁提取物调控cAMP-PKA通路影响小鼠体长增长及大鼠慢波睡眠的具体机制。方法 (1)48只昆明种雄性小鼠随机分为6组,分别为空白对照组(灌胃去离子水,并于末次灌胃后侧脑室注射灭菌的0.9%NaCl溶液)、蓝墨水组(灌胃去离子水,并于末次灌胃后侧脑室注射过滤灭菌的蓝墨水)、用药组(灌胃酸枣仁提取物水溶液,并于末次灌胃后侧脑室注射过滤灭菌的蓝墨水)、阳性对照组(灌胃酸枣仁皂苷A水溶液,并于末次灌胃后侧脑室注射过滤灭菌的蓝墨水)、Gi/o蛋白抑制剂+用药组(灌胃酸枣仁提取物水溶液,并于末次灌胃后侧脑室注射加有过滤灭菌蓝墨水的PT)和5-HT1AR抑制剂+用药组(灌胃酸枣仁提取物水溶液,并于末次灌胃后侧脑室注射加有过滤灭菌蓝墨水的P-MPPF)。各组小鼠灌胃相应药物,连续20 d。测定小鼠体长差异,采用ELISA法测定小鼠血清生长激素(GH)水平、脑组织5-羟色胺1A受体(5-HT1AR)活性、PKA的含量和Gi/o蛋白的含量;Western blot法测定小鼠脑组织5-HT1AR蛋白的表达。(2)36只SD大鼠随机分成6组,分组及给药方式同小鼠,连续给药3 d后,采用WXL睡眠自动分析系统,分析大鼠睡眠时相差异。结果 (1)与空白对照组和蓝墨水组比较,用药组、Gi/o蛋白抑制剂+用药组、5-HT1AR抑制剂+用药组、阳性对照组小鼠体长均明显增长(P<0.01);与空白对照组比较,用药组和阳性对照组小鼠血清GH水平、5-HT1AR蛋白含量、Gi/o蛋白含量均明显升高(P<0.01),5-HT1AR活性升高(P<0.05),PKA含量明显降低(P<0.01);与蓝墨水组比较,用药组和阳性对照组小鼠血清GH水平明显升高(P<0.01),Gi/o蛋白含量、5-HT1AR活性升高(P<0.05),PKA含量明显降低(P<0.01);与Gi/o蛋白抑制剂+用药组和5-HT1AR抑制剂+用药组比较,用药组和阳性对照组小鼠血清GH水平、5-HT1AR蛋白含量均明显升高(P<0.01),5-HT1AR活性升高(P<0.05)。用药组与阳性对照组比较,小鼠�Objective To explore the mechanism of Ziziphi Spinosae Semen extract affecting the mice growth in length and slow wave sleep of rats by regulating the cAMP-PKA pathway. Methods(1)Forty-eight Kunming male mice were randomly divided into 6 groups: blank control group(intragastrically administered with deionized water, and injected with sterilized 0.9% NaCl in the lateral ventricle after the last intragastric administration),blue ink group( intragastrically administered with deionized water,and injected with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration), medication group( intragastric administration of the Ziziphi Spinosae Semen extract,and injection of filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration),positive control group( intragastric administration of jujube saponin A,and injection of filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration),Gi/o protein inhibitor + medication group( intragastric administration of Ziziphi Spinosae Semen extract,and injection of PT with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration) and 5-HT1 AR inhibitor + medication group( intragastric administration of Ziziphi Spinosae Semen extract,and injection of P-MPPF with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration). The mice in each group were given corresponding drugs by intragastric administration for 20 days. The body length of the mice was measured. The level of serum growth hormone( GH),the activity of 5-hydroxytryptamine 1 A receptor( 5-HT1 AR) in brain tissue,the contents of PKA and Gi/o protein in brain tissue were measured by ELISA. The expression of 5-HT1 AR protein in mouse brain tissue was determined by Western blot.(2)Thirty six SD rats were randomly divided into 6 groups( 6 rats in each group). The grouping and administration methods were the same as those of mic

关 键 词：酸枣仁提取物 酸枣仁皂苷A 骨骼增长 cAMP-PKA通路 Gi/o蛋白 5-HT1A受体 模型小鼠 模型大鼠 慢波睡眠 

分 类 号：R285.5[医药卫生—中药学]