热激处理的西瓜未受精胚珠转录组分析  

Transcriptome Analysis of the Unfertilized Ovule of Watermelon with Heat Shock Treatment

作　　者：朱迎春[1] 李卫华[1] 安国林[1] 袁高鹏 司文静 刘君璞[1] 孙德玺[1] ZHU Ying-chun;Ll Wei-hua;AN Guo-lin;YUAN Gao-peng;Sl Wen-jing;LIU Jun-pu;SUN De-xi(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《植物遗传资源学报》2021年第3期834-850,共17页Journal of Plant Genetic Resources

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-2020-ZFRI);郑州市重大科技专项(188PCXZX802);现代农业产业技术体系(CARS-26);河南省重点科技项目(202102110194)。

摘　　要：为揭示热激影响西瓜未受精胚珠膨大的分子机理,本研究以中果型西瓜品种中科6号为试材,将当天开花的西瓜未授粉子房于37℃培养箱中进行热激处理,分别对热激处理0 h(A1)、4 h(A3)、8 h(A5)、12 h(A7)和24 h(A8)的样品取样,提取RNA并进行转录组测序。4个比较组(A3A1、A5A1、A7A1和A8A1)共检测到10093个差异表达基因(DEGs),其中共有的DEGs为4645个。DEGs的聚类分析结果表明,37℃热激处理4 h时,未受精胚珠内部转录机制发生了剧烈变化,西瓜未受精胚珠的热激分子响应可能主要发生在热激处理前期。GO分析发现DEGs主要在细胞加工、细胞元件和细胞器等方面得到注释,暗示热激处理可能增加了子房细胞的活跃程度。DEGs的KEGG分析结果显示氨基酸代谢、碳水化合物和糖代谢、植物激素信号转导、MAPK信号转导通路等得到富集,它们可能对西瓜未受精胚珠发育产生了较大影响。另外,研究发现,当西瓜未受精胚珠在热激的诱导下,热激蛋白、植物激素和胚胎发育相关基因的表达变化可能是诱导未受精胚珠发育的重要因素,这些结果为进一步解析西瓜未受精胚珠热激发育的分子机制提供了思路。In order to reveal the molecular mechanism of heat shock on ovule expansion,the global transcriptional profiles using RNA-seq were revealed based on the unfertilized ovules of watermelon under heat shock treatment. Total RNAs were extracted from the unfertilized ovules at 0 hour(A1),4 hour(A3),8 hour(A5),12 hour(A7)and 24 hour(A8)post heat shock at 37 ℃,and then subjected for RNA-seq. Overall,10093 differentially expressed genes(DEGs)were detected in the four pair-wise comparison groups(A3 to A1,A5 to A1,A7 to A1 and A8 to A1),including 4645 DEGs that were commonly detected at four comparisons. The cluster analysis of DEGs showed that the transcriptional reprograming of unfertilized ovules occurred after heat shock at 37 ℃ for 4 h,implying that the earlier response upon heat shock treatment. GO analysis showed that DEGs were mainly annotated in cell processes,cell parts and organelles,suggesting that heat shock may increase the activity of ovule cells. KEGG analysis revealed enrichments in amino acid metabolism,carbohydrate and sugar metabolism,plant hormone signal transduction and MAPK signaling pathway,which raises a putative impact on the development of unfertilized ovule. In addition,it was found that changes in the expression of DEGs related to heat shock proteins,plant hormones and embryo development under heat stress may be important in inducing unfertilized ovule development,which provides preliminary insights for future unlocking the molecular mechanism of heat shock development of unfertilized ovules in watermelon.

关 键 词：西瓜 未受精胚珠 热激 比较转录组 

分 类 号：S651[农业科学—果树学]