温州芜菁与水果紫苤蓝杂交创制芸薹属异源四倍体杂种  

Production of Synthesized Brassica Allotetraploids between Wenzhou Turnip And Purple Kohlrabi

作　　者：金平 朱志玉 张靓 傅敏杰 郑文 张萼 陈加红[2] 吴建国 朱祝军 JING Ping;ZHU Zhi-yu;ZHANG Liang;FU Min-jie;ZHENG Wen;ZHANG E;CHEN Jia-hong;WU Jian-guo;ZHU Zhu-jun(College of Agriculture and Food Science,Zhejiang A&F University/Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,Hangzhou 311300;Department of Health and Agriculture,Hangzhou Wanxiang Polytechnic,Hangzhou 310023)

机构地区：[1]浙江农林大学农业与食品科学学院/浙江省农产品品质改良技术研究重点实验室,杭州311300 [2]杭州万向职业技术学院健康与现代农业系,杭州310023

出　　处：《植物遗传资源学报》2021年第3期851-859,共9页Journal of Plant Genetic Resources

基　　金：浙江省省级现代种业发展工程(2015004);浙江省“三农六方”项目(2045210244)。

摘　　要：本研究以温州芜菁(Brassica rapa L. em. Matzg.,2n=20,AA)为母本,水果紫苤蓝(B. oleracea L. var. caulorapa DC,2n=18,CC)为父本进行远缘杂交,创造种间杂种。通过花期剥蕾授粉、子房培养共获得2株杂种,经扩繁得到无性苗19株,经形态学分析、细胞学观察和RAPD分子鉴定出2株均为温州芜菁×紫苤蓝的真杂种,后利用染色体加倍获得异源四倍体新型芜菁甘蓝新种质材料(AACC,2n=38)。两杂种株系出现茎色分离,根部与茎部均有膨大,但程度低于亲本。异源四倍体的花器官较大,花药发育正常,可育花粉率高。本研究所创制的异源四倍体新种质,在一定程度上拓展了芸薹属蔬菜种质资源的遗传基础。In the present study,wide hybridization was performed between Whenzhou turnip(Brassica rapa L. em. Matzg.,2 n=20,AA)and purple kohlrabi(B. oleracea L. var. caulorapa DC,2 n=18,CC)using hand emasculation,followed by the tissue culture for hybrid seeds. A total of two hybrids were obtained through bud pollination and ovary culture. Morphological analysis,cytological observation and RAPD molecular analysis further confirmed the two strains as hybrids of Wenzhou turnip × purple kohlrabi. Nineteen asexual seedlings were obtained through expanding propagation,and the novel allotetraploid rutabagas germplasm was obtained by chromosome doubling(AACC,2 n=38). The asexual plants derived from two hybrids were different on stem color,and enlarged roots and stems,lower than those of the parents. The allotetraploids represented larger floral organs,higher pollen viabilities and anthers developed as expected. The new allotetraploid generated from this study provided possibility on enriching the genetic diversity of Brassica germplasm resources.

关 键 词：温州芜菁 水果紫苤蓝 芜菁甘蓝 种间杂种 异源四倍体 

分 类 号：S631.3[农业科学—蔬菜学] S635.9[农业科学—园艺学]