超声刺激对大鼠前额叶皮层ECoG的调控  

Ultrasound Stimulation on ECoG in Rat Prefrontal Cortex

作　　者：王君 随力[1] 吴永亮 郑政[1] Wang Jun;Sui Li;Wu Yongliang;Zheng Zheng(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《中国生物医学工程学报》2021年第2期195-201,共7页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(11179015);上海理工大学科技发展项目(16KJFZ107,2017KJFZ160)。

摘　　要：超声刺激具有神经调控功能,探讨其对前额叶皮层的神经调控作用及机制。超声刺激经颅施加到麻醉状态下大鼠(n=15)的前额叶皮层(刺激持续20 min),并于刺激前、刺激中和超声刺激后分别记录前额叶皮层的皮层电位(ECo G)。ECo G的特征参数分别为总功率谱密度(PSD)、4个频段(δ:0.5~4 Hz,θ:4~8 Hz,α:8~13 Hz,β:13~30 Hz)的PSD、ECo G的平均幅值及4个频段的平均幅值。为弱化实验大鼠之间的个体差异,并且能揭示超声刺激的时间-效应关系,超声刺激中和刺激后的ECo G的特征参数值以相对于刺激前的百分比形式来进行统计学比较和分析,并得出实验结果。1)ECo G总PSD:整个超声刺激过程中,分为7个时间段,分别为超声刺激的0~1、1~2、2~3、3~4、4~5、5~10、10~20 min,施加超声刺激0~1 min,前额叶皮层ECo G的总PSD为刺激前的139.2%±13.2%(x珋±SEM),和刺激前相比差异具有显著性(P<0.05);其他6个时间段的ECo G的总PSD和刺激前相比差异均具有显著性(P<0.05)。超声刺激停止后5 min以内,ECo G的PSD为刺激前的90.1%±9.9%,和刺激前相比差异无显著性(P>0.05)。2)4个频段的PSD:超声刺激对4个频段的PSD均具有增强效应,超声刺激中4个频段的PSD和刺激前相比差异均具有显著性(P<0.05);超声刺激停止后,4个频段的PSD和刺激前相比差异无显著性(P>0.05)。3)ECo G的平均幅值:超声刺激中的ECo G的平均幅值和刺激前相比差异具有显著性(P<0.05);超声刺激停止后,ECo G的平均幅值和刺激前相比差异无显著性(P>0.05)。4)4个频段的平均幅值:超声刺激可增强4个频段的平均幅值,对δ频段的影响最为明显,影响时程达10 min,对θ、α和β频段的影响时程小于5 min。超声刺激对大鼠前额叶皮层的ECo G具有调控作用,这种调控具有及时性及快速恢复的特点。Ultrasound stimulation has neuromodulatory function.In the present study,we explored the neuromodulatory effect and mechanisms of ultrasound stimulation on prefrontal cortex.Ultrasound stimuli were applied transcranially to the prefrontal cortex of anesthetized rats(n=15)and electrocorticogram(ECoG)was recorded before,during(20 min)and after the ultrasound stimulation.The characteristic parameters of ECoG included the total power spectral density(PSD),the PSD of four frequency bands(δ:0.5~4 Hz,θ:4~8 Hz,α:8~13 Hz,β:13~30 Hz),the average amplitude of ECoG and the average amplitude of the four frequency bands.To attenuate individual differences between experimental rats and reveal the time-effect relationship of ultrasound stimulation,the characteristic parameter values of ECoG during and after ultrasound stimulation were statistically compared and analyzed in the form of percentages relative to pre-stimulation(defined as 100%).The results could be described from four aspects.1)The whole ultrasound stimulation process was divided into 7 time periods,which were 0~1,1~2,2~3,3~4,4~5,5~10,10~20 min of ultrasound stimulation,respectively.The total PSD of ECoG was 139.2±13.2%in prefrontal cortex within 1 min of the applied ultrasound stimulation,and the difference was significant compared with that before the stimulation(P<0.05).The differences between the total PSD of ECoG in the other 6 time periods compared to pre-stimulation were all significant(P<0.05).Within 5 min after the cessation of ultrasound stimulation,the PSD of ECoG was 90.1±9.9% of that before stimulation,and the difference was not significant compared with that before stimulation(P>0.05).2)Ultrasound stimulation had an enhancing effect on PSD in all four frequency bands,and the difference between PSD in all four frequency bands in ultrasound stimulation and before stimulation was significant(P<0.05).After the cessation of ultrasound stimulation,there was no significant difference between the PSD of the four frequency bands compared with that before

关 键 词：超声刺激 皮层电位(ECoG) 前额叶皮层 神经调控 

分 类 号：R318[医药卫生—生物医学工程]