过敏者外周血DNA甲基化差异  

DNA Methylation Differences in Peripheral Blood of Patients with Anaphylaxis

作　　者：刘明哲 郭华林 冯彦[2] 李建国[3] 李鹏飞 高彩荣[1] 郭相杰[1] LIU Ming-zhe;GUO Hua-lin;FENG Yan;LI Jian-guo;LI Peng-fei;GAO Cai-rong;GUO Xiang-jie(School of Forensic Medicine,Shanxi Medical University,Jinzhong 030600,Shanxi Province,China;First Hospital of Shanxi Medical University,Taiyuan 030000,China;China Institute for Radiation Protection,Taiyuan 030000,China)

机构地区：[1]山西医科大学法医学院,山西晋中030600 [2]山西医科大学第一医院,山西太原030000 [3]中国辐射防护研究院,山西太原030000

出　　处：《法医学杂志》2021年第2期211-214,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金面上项目(81971790);山西省科技厅自然科学基金面上项目(201601D102070);山西医科大学博士启动基金资助项目(03201536)。

摘　　要：目的通过研究头孢类药物过敏性休克死亡者外周血有核细胞DNA甲基化情况,为药物过敏性休克死亡的法医学诊断提供新的研究方向和依据。方法运用甲基化芯片检测头孢类药物过敏性休克死亡者和免疫正常者外周血有核细胞DNA甲基化情况。使用R语言methylkit、ChAMP包分别对测序数据、芯片数据进行DNA甲基化差异分析,运用随机森林算法对所得DNA甲基化差异位点重要性进行评估。结果在ETS1、PRR23B、GNAS等基因座上获得与头孢类药物过敏高度相关的DNA甲基化差异位点。结论头孢类药物过敏与DNA甲基化相关,DNA甲基化有望成为过敏性休克死亡的法医学鉴定新策略。Objective To study the DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and to provide a new research direction and basis for the forensic diagnosis of shock caused by drug hypersensitiveness.Methods Methylation microarray was used to detect DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and normal subjects.Sequencing data and chip data were analyzed for differences in DNA methylation using R language methylkit,ChAMP package.Random forest algorithm was used to evaluate the importance of the DNA methylation differential sites.Results Differential sites of DNA methylation highly associated with anaphylaxis caused by cephalospo-rin drugs were obtained at loci such as ETS1,PRR23B and GNAS.Conclusion Cephalosporin allergy is associated with DNA methylation,and DNA methylation may be a new strategy for forensic identifi-cation of anaphylactic shock and death.

关 键 词：法医病理学 过敏反应 DNA甲基化 DNA芯片 药物过敏 

分 类 号：DF795.1[医药卫生—法医学]