产羟基喹啉基乙酮芽胞杆菌的筛选与分析  

Screening and Analysis of Bacillus Producing Quinacetol

作　　者：陈峥[1] 刘波[1] CHEN Zheng;Liu Bo(Institute of Agricultural Biological Resources,Fujian Academy ofAgricultural Sciences,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业科技》2021年第4期9-15,共7页Fujian Agricultural Science and Technology

基　　金：福建省科技计划项目——省属公益类科研院所基本科研专项(2019R1034-4)。

摘　　要：芽胞杆菌是一类重要的生防微生物,能产生多样的抗菌物质。为了挖掘产喹啉类抑菌物质的菌株资源,以56个芽胞杆菌菌株为试验材料,运用液相色谱四级杆飞行时间质谱(LC-QTOF-MS)技术对其发酵产物进行检测,对质谱数据进行分子特征提取和Metlin谱库比对。结果表明:筛选获得6株产羟基喹啉基乙酮(Quinacetol)的芽胞杆菌,分别是土壤芽胞杆菌FJAT 14232(Bacillus soli)、枯草芽胞杆菌FJAT 8784(Bacillus subtilis)、波茨坦短芽胞杆菌FJAT 10006(Brevibacillus borstelensis)、浸麻类芽胞杆菌FJAT 14204(Paenibacillus macerans)、嗜碱芽胞杆菌FJAT 10014(Bacillus alcalophilus)和米氏解硫胺素芽胞杆菌FJAT 14205(Aneurinibacillus migulanus)。6株芽胞杆菌中的羟基喹啉基乙酮占各自总代谢物的相对含量均高于1.17%,且与Metlin谱库匹配得分均大于95.35。其中,土壤芽胞杆菌FJAT 14232(Bacillus soli)中的羟基喹啉基乙酮相对含量最高,占其总代谢物相对含量的1.68%。嗜碱芽胞杆菌FJAT 10014(Bacillus alcalophilus)的谱库检索得分最高,为99.13。本研究为微生物来源的喹啉类抑菌剂的开发与利用提供菌种资源和数据支持。Bacillus is an important biocontrol microorganism which can produce a variety of antimicrobial substances.In order to mine the strain resources producing the antibacterial substance quinolines,a total of 56 Bacillus strains were used as the experimental materials and their fermentation products were detected by the liquid chromatography-quadrupole time of flight-mass spectrometry(LC-QTOF-MS),and the molecular characteristics were extracted from the mass spectrometric data and compared with the Metlin library.The results showed that,6 strains of Bacillus producing quinacetol were screened and obtained,including Bacillus soli FJAT 14232,Bacillus subtilis FJAT 8784,Brevibacillus borstelensis FJAT 10006,Paenibacillus macerans FJAT 14204,Bacillus alcalophilus FJAT 10014,Aneurinibacillus migulanus FJAT 14205.The relative contents of quinacetol in the total metabolites of the six Bacillus strains were all higher than 1.17%,and the matching scores with Metlin library were all greater than 95.35.Among them,the relative content of quinacetol in Bacillus soli FJAT 14232 was the highest,accounting for 1.68%of the relative content of its total metabolite,and Bacillus alcalophilus FJAT 10014 had the highest score of 99.13 by library searching.This study could provide the strain resources and data support for the development and utilization of the quinoline bacteriostatic agent from microorganisms.

关 键 词：羟基喹啉基乙酮 喹啉类 芽胞杆菌 抗菌化合物 液相色谱四级杆飞行时间质谱 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]