大气细颗粒物PM_(2.5)中16种多环芳烃检测方法研究  

Research of detection methods for 16 kinds of PAHs in atmospheric fine particulate matter PM_(2.5)

作　　者：周浩丰[1] 李四生[1] 葛成相[1] 李昌安[1] ZHOU Hao-feng;LI Si-sheng;GE Cheng-xiang;LI Chang-an(Hefei Center for Disease Control and Prevention,Anhui 230061,China)

机构地区：[1]合肥市疾病预防控制中心理化检验科,安徽230061

出　　处：《安徽预防医学杂志》2021年第2期85-90,共6页Anhui Journal of Preventive Medicine

基　　金：中央财政资金补助雾霾监测专项;合肥市医学重点学科建设资助。

摘　　要：目的探索利用改进PM_(2.5)中16种多环芳烃高效液相色谱法测定合肥市大气细颗粒物PM_(2.5)中多环芳烃含量。方法取采集空气后滤膜1/4剪碎,乙腈超声提取,高效液相色谱法测定,改用多环芳烃(Polycyclic aromatic hydrocarbons,PAHs)专用柱(4.60 mm×250 mm,5.0μm),流动相为水和乙腈,流速1.5 mL/min,梯度洗脱,PDA检测器和FLR检测器同时检测。结果16种PAHs空白加标回收率在72%~105%之间,标准曲线线性方程相关系数r>0.999,相对标准差(RSD)0.61%~7.72%,方法检出限为0.004~0.023 ng/m^(3),定量下限为0.014~0.094 ng/m^(3)(采样量为144 m^(3))。结论改进过后的方法在检测合肥市大气颗粒物PM_(2.5)中具有简便、快速、灵敏等优点,提高了低环数PAHs回收率和PAHs分离效果,具有较高的应用价值。Objective To develop an improved high performance liquid chromatography(HPLC)method for the determination of 16 kinds of polycyclic aromatic hydrocarbons(PAHs)in PM_(2.5) in Hefei.Methods After collecting air,1/4 of filter membrane was cut up,acetonitrile was added for ultrasonic extraction,and determined by HPLC method.The special column for PAHs(4.60 mm×250 mm,5.0μm)was used,the mobile phase was water and acetonitrile,the flow rate was 1.5 ml/min,gradient elution method was used,PDA detector and FLR detector were detected at the same time.Results The blank spike recovery rates of 16 kinds of PAHs ranged from 72%~105%.The correlation coefficient of linear equation of standard curve was r>0.999.The relative standard deviation(RSD)was 0.61%~7.72%.The detection limit was 0.004~0.023 ng/m^(3),and the lower limit of quantification was 0.014~0.094 ng/m^(3)(sampling volume was 144 m^(3)).Conclusion The improved method has the advantages of simplicity,rapidity and sensitivity in the detection of PM_(2.5) in the atmosphere.It improves the recovery rate of PAHs with low ring number and the separation effect of PAHs,and has high application value.

关 键 词：大气 细颗粒物PM_(2.5) 多环芳烃 超声萃取 超高效液相色谱 

分 类 号：R122[医药卫生—环境卫生学]