芪红通络颗粒对大鼠脑缺血再灌注损伤保护作用及其机制研究  

Protective Effect of Qihong Tongluo Granule Against Cerebral Ischemia/Reperfusion Injury in Rats and Its Mechanism

作　　者：高玉菊 杨满琴[1] 王丽[1] 罗胜勇[2] Gao Yu-ju;YANG Man-qin;WANG Li;LUO Sheng-yong(The Second Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230061,China;Anhui Academy of Medical Sciences,Anhui Hefei 230061,China)

机构地区：[1]安徽中医药大学第二附属医院,安徽合肥230061 [2]安徽省医学科学研究院,安徽合肥230061

出　　处：《安徽中医药大学学报》2021年第3期64-69,共6页Journal of Anhui University of Chinese Medicine

基　　金：安徽省高等学校自然科学研究项目(KJ2019A0446);安徽省自然科学基金(1608085MH161)。

摘　　要：目的观察芪红通络颗粒对大鼠脑缺血再灌注损伤的保护作用,并初步探讨其作用机制。方法将SD大鼠随机分为假手术组,模型组,尼莫地平组,芪红通络颗粒高、中、低剂量(10.8、5.4、2.7 g/kg)组,采用线栓法建立大鼠局灶性脑缺血再灌注损伤模型。各组大鼠术前连续3 d、术后连续2 d灌胃给药,每日1次。术后72 h采用Longa法进行神经功能缺损评分,TTC染色法测定脑梗死体积,HE染色观察脑组织病理学改变,比色法测定脑组织丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)活力,Western blot法测定脑组织核因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)和血红素氧合酶-1(heme oxygenase 1,HO-1)蛋白表达水平。结果与模型组比较,芪红通络颗粒高、中、低剂量组脑梗死体积显著缩小(P<0.05);高、中剂量组神经行为学障碍症状明显减轻(P<0.05),脑组织病理改变明显减轻;高、中、低剂量组脑组织中MDA含量明显降低,SOD和GSH-Px活力明显增加(P<0.05);高、中剂量组脑组织中Nrf2和HO-1蛋白表达水平明显升高(P<0.05)。结论芪红通络颗粒对大鼠脑缺血再灌注损伤具有较好的保护作用,其机制可能与其激活Nrf2/HO-1信号通路,抑制氧化应激反应,从而产生抗氧化作用有关。Objective To investigate the protective effect of Qihong Tongluo Granule against cerebral ischemia/reperfusion injury in rats and its preliminary mechanism of action.Methods Sprague-Dawley rats were randomly divided into sham-operation group,model group,nimodipine group,and high-,middle-,and low-dose(10.8,5.4,and 2.7 g/kg)Qihong Tongluo Granule groups.The suture method was used to establish a rat model of focal cerebral ischemia/reperfusion injury.The rats were administered once a day by gavage for 3 consecutive days before surgery and 2 consecutive days after surgery.At 72 hours after surgery,the Longa method was used to determine neurological deficit score;TTC staining was used to measure cerebral infarct volume;HE staining was used to observe the pathological changes of brain tissue;colorimetry was used to measure the content of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in brain tissue;Western blot was used to measure the protein expression levels of nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)in brain tissue.Results Compared with the model group,the high-,middle-,and low-dose Qihong Tongluo Granule groups had a significant reduction in cerebral infarct volume(P<0.05);the high-and middle-dose Qihong Tongluo Granule groups had significantly alleviated symptoms of neurobehavioral disturbance(P<0.05)and pathological changes of brain tissue;the high-,middle-,and low-dose Qihong Tongluo Granule groups had a significant reduction in the content of MDA and significant increases in the activities of SOD and GSH-Px in brain tissue(P<0.05);the high-and middle-dose Qihong Tongluo Granule groups had significant increases in the protein expression levels of Nrf2 and HO-1 in brain tissue(P<0.05).Conclusion Qihong Tongluo Granule exerts a good protective effect against cerebral ischemia/reperfusion injury in rats,possibly by activating the Nrf2/HO-1 signaling pathway,inhibiting oxidative stress response,and thus producing an antioxidant

关 键 词：芪红通络颗粒 缺血再灌注 氧化应激 核因子E2相关因子2 血红素氧合酶-1 

分 类 号：R285.5[医药卫生—中药学]