Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives  

作　　者：Yu Xin Qiuyue Gao Yu Gu Mengyao Hao Guangming Fan Liang Zhang 

机构地区：[1]Key Laboratory of Industry Biotechnology(Ministry of Education),School of Biotechnology,Jiangnan University,Wuxi 214122,China [2]National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China

出　　处：《Frontiers of Chemical Science and Engineering》2021年第3期615-629,共15页化学科学与工程前沿（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2018YFA0900304-300);the Natural Science Foundation of Jiangsu Province(Grant Nos.BK20160053 and BE2018055);the Priority Academic Program Development of Jiangsu Higher Education Institutions,111 Project(Grant No.111-2-06).

摘　　要：A cholesterol oxidase(COD)was hybridized with Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+).After precipitation with PO_(4)^(3-) at 4℃ for 72 h,the resulting pellets were freeze-dried.In scanning electron microscopy assays,the metal-COD complexes revealed flower-like or granular structures after hybridization.Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials.X-ray diffraction analysis indicated that COD was encapsulated in CaHPO_(4)·2H_(2)O-,Zn_(3)(PO_(4))_(2)·4H_(2)O-,AlPO_(4-),FeP_(4-) and Mn_(3)(PO_(4))_(2)·3H_(2)O-based nanostructures,respectively.Differential scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5℃ to 167.02℃,167.02℃,137.70℃,172.85℃ and 160.99℃,respectively.Using steroid derivatives as substrates,this enzyme could convert cholesterol,pregnenolone,dehydroepiandrosterone,ergosterol,b-sitosterol and stigmasterol to related single products.Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme.In addition,substrate selectivity could be affected by various metal materials.Briefly,using Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+) as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures,and could help to promote the stability and tolerant properties of the enzyme,while also enhancing its catalytic characteristics.

关 键 词：cholesterol oxidase metal-enzyme hybridization nanostructures sterol derivatives bioconversion 

分 类 号：O62[理学—有机化学]