猪脱细胞真皮基质影响蛋白激酶B/β-连环蛋白信号通路小鼠创面的研究  

Effects of porcine acellular dermal matrix on the expression ofβ-catenin and protein kinase B during mouse wounds healing at different time points

作　　者：郑洁[1] 夏稳伸 李炳辉[1] 李恭驰[2] 李斌斌 邹利军[1] 冯自波[1] 杜烨 王知 张晨晨 邝莉雯 杨鸿[1] Zheng Jie;Xia Wenshen;Li Binghui;Li Gongchi;Li Binbin;Zou Lijun;Feng Zibo;Du Ye;Wang Zhi;Zhang Chenchen;Kuang Liwen;Yang Hong(Department of Wound Repair,Wuhan Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China;Department of Hand Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属梨园医院创面修复科,武汉430077 [2]华中科技大学同济医学院附属协和医院手外科,武汉430022

出　　处：《中华实验外科杂志》2021年第5期825-828,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年科学基金(81801922);国家重大疾病多学科合作诊疗能力建设项目(国卫办医函[2019]542号);湖北省自然科学基金面上项目(2018CFB756);湖北省科技创新平台专项资助建设项目(2018BCC34C);湖北省科技平台专项立项项目(2020BCB029)。

摘　　要：目的观察猪脱细胞真皮基质(pADM)在小鼠创面修复过程中对蛋白激酶B(Akt)及β-连环蛋白(β-catenin)表达水平的影响。方法猪皮经脱细胞处理后制成微粒皮,15只C57BL/L小鼠在脊柱左右各制作直径为6 mm的圆形皮肤缺损,左侧以纱布覆盖,常规护理,右侧以pADM覆盖,不作特别处理,按处理方法分为pADM组(实验组)及纱布组(对照组),建模后第1、2、3、4、6周每次取3只小鼠处死,并取创面组织,使用免疫组织化学方法检测Akt及β-catenin在组织中的表达。组间比较采用t检验。结果在模型建立后的第1、2、3、4、6周,实验组各时间点AKT蛋白表达量两两比较差异均有统计学意义(4022.02±652.42、1160.12±225.60、3422.17±675.91、324.15±64.67、27.26±4.49,t1-2=9.458、t2-3=-4.839、t3-4=8.776、t4-6=7.436,P<0.05)。同时对照组蛋白表达在各时间点间有统计学意义(1110.98±219.14、337.13±61.37、118.80±18.08、834.98±64.19、395.70±76.79,t1-2=8.802、t2-3=4.951、t3-4=-19.584,t4-6=6.835,P<0.05)。第2、3、4、6周时实验组(1160.12±225.60、3422.17±675.91、324.15±64.67、27.26±4.49)及对照组(337.13±61.37、118.80±18.08、834.98±64.19、395.70±76.79)的蛋白表达差异有统计学意义(t2=8.574、t3=8.694、t4=-10.183、t6=-7.880,P<0.05),其他组差异无统计学意义(4022.02±652.42、1160.12±225.60,t1=-0.363,P>0.05)。实验组各时间点β-catenin蛋白表达量两两比较差异均有统计学意义(3646.32±687.04、8447.29±1181.79、9488.65±1439.52、6158.74±1083.52,t1-2=-6.626、t3-4=12.101,P<0.05),对照组蛋白表达在各时间点间有统计学意义(4364.09±811.29、2187.85±424.84、2752.27±500.01、7522.95±1109.30、2592.14±474.23,t1-2=-4.334、t3-4=-5.594、t4-6=12.154,P<0.05),实验组(8447.29±1181.79、9488.65±1439.52)及对照组(2187.85±424.84、2752.27±500.01)两组间的β-catenin的蛋白表达在第2、3周时差异有统计学意义(t2=9.351、t3=-9.225,P<0.05),其他组差异无统计学意义(3646.Objective To investigate the effect of porcine acellular dermal matrix(pADM)on the expression ofβ-catenin and protein kinase B(Akt)during mouse wounds healing at different time points after skin injury.Methods The skin injury models were established in 15 C57BL/6 mice.The spine was set as the midline,and on its both sides a full-thickness skin defect wound with 6 mm diameter was made.The left wound was covered with gauze(control group),and the right wound was covered with particulate pADM(pADM group),and the tissues of wound edge were collected at the 1st,2nd,3rd,4th,and 6th week after the establishment of the model.The expression ofβ-catenin and Akt proteins was detected by immunohistochemical staining,and that of the two kind proteins in tissues and cells was observed pathologically.T test was used for comparison between groups.Results Immunohistochemical detection of protein expression in mouse wound tissue showed that the protein expression levels ofβ-catenin and Akt were different in the two groups,and all of them had statistical significance(respectively).For the expression of Akt protein,there was significant difference in pADM between the two groups,significant differences between 1st week and 2nd week,2nd week and 3rd week,3rd week and 4th week,4th week and 6th week(4022.02±652.42,1160.12±225.60,3422.17±675.91,324.15±64.67,27.26±4.49,t1-2=9.458,t2-3=-4.839,t3-4=8.776,t4-6=7.436,P<0.05),no significant differences between the other groups,in control group,significant differences between 1st week and 2nd week,2nd week and 3rd week,3rd week and 4th week,4th week and 6th week(1110.98±219.14,337.13±61.37,118.80±18.08,834.98±64.19,395.70±76.79,t1-2=8.802,t2-3=4.951,t3-4=-19.584,t4-6=6.835,P<0.05),significant difference existed at the 2nd,3rd and 6th week between pADM group(1160.12±225.60,3422.17±675.91,324.15±64.67,27.26±4.49)and control group(337.13±61.37,118.80±18.08,834.98±64.19,395.70±76.79,t2=8.574,t3=8.694,t4=-10.183,t6=-7.880,P<0.05),but no significant difference was found at the 1st

关 键 词：脱细胞真皮基质 蛋白激酶B Β-连环蛋白 创面 

分 类 号：R641[医药卫生—外科学]