脐带间充质干细胞在小鼠体内的示踪研究  

Tracing of umbilical cord mesenchymal stem cells in mice

作　　者：刘广芝[1,2] 杜晗 袁蒙蒙 张永辉 刘梁 杨秋云 Liu Guangzhi;Du Han;Yuan Mengmeng;Zhang Yonghui;Liu Liang;Yang Qiuyun(International Medical Center,Henan Provincial People’s Hospital,Zhengzhou 450003,China;Henan Key Laboratory of Stem Cell Differentiation and Modification,Zhengzhou 450003,China;Department of Gynecology,Henan Provincial People’s Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院国际医疗中心,郑州450003 [2]河南省干细胞分化与调控重点实验室,郑州450003 [3]河南省人民医院妇科,郑州450003

出　　处：《中华实验外科杂志》2021年第5期901-903,共3页Chinese Journal of Experimental Surgery

摘　　要：目的比较通过卵巢局部注射和尾静脉注射两种不同的移植方式,脐带间充质干细胞(UCMSCs)在卵巢损伤小鼠体内的分布、迁移和增殖,探讨最佳移植途径。方法取8周龄ICR雌性小鼠12只,采用环磷酰胺(CTX)200 mg/kg+白消安(BUS)30 mg/kg一次性腹腔注射方式建立卵巢损伤小鼠模型;采用慢病毒转染途径构建标记有荧光素酶(Luc)的UCMSCs;将卵巢损伤小鼠随机分为两组,分别采用尾静脉移植(2×106个细胞)和单侧卵巢局部移植(2×105个细胞),移植后采用小动物活体成像(IVIS)技术示踪UCMSCs在小鼠体内的分布、迁移及增殖。组间比较采用t检验。结果化疗药物处理后1周小鼠血清FSH水平[(17.971±0.311)μg/L]显著高于化疗药物处理前[(14.420±0.622)μg/L]、化疗药物处理后1周小鼠血清E2水平[(320.321±8.682)ng/L]显著低于化疗药物处理前[(175.383±19.400)ng/L],差异有统计学意义(t=5.106、4.800,P<0.05);卵巢局部移植Luc-UCMSCs后在小鼠卵巢部位可观察到荧光信号(d1:2.61×105±0.33、d2:1.35×105±0.23、d3:3.09×105±0.29、d4:4.12×105±0.43),其余部位未见荧光信号,并且在移植第2天开始荧光信号逐渐增强,差异有统计学意义(t=7.011、8.311、4.562,P<0.05),第5天信号消失,而尾静脉移植组小鼠在移植后7 d内各部位均未见到明显荧光信号。结论UCMSCs通过卵巢局部注射可以在小鼠受损的卵巢局部聚集并存活,与尾静脉注射比较,卵巢局部注射可能会取得更好的治疗效果。Objective To find the optimal transplantation route by comparing the local ovarian injection and tail vein injection,and the distribution,migration and proliferation of umbilical cord mesenchymal stem cells(UCMSCs)in mice models of ovarian injury via thein vivo imaging system.Methods A total of 200 mg/kg cyclophosphamide and 30 mg/kg busulfan were injected intraperitoneally to establish the mice models of ovarian injury.Lentiviral transfection was done to construct UCMSCs labeled with luciferase(Luc).The mice models of ovarian injury were randomly divided into two groups:tail vein injection group(2×106 cells)and unilateral ovarian local injection group(2×105 cells).The distribution,migration and proliferation of UCMSCs in the mice models of ovarian injury were observed by thein vivo imaging system.Results Luc-UCMSCs injected unilaterally into the ovary showed a gradual increase in the fluorescence signal on the second day to the fourth day after transplantation,and no fluorescence signal was found in the other parts of the body.On the fifth day after transplantation,the fluorescence signal disappeared.In the tail vein injection group,fluorescence signals were not observed in all parts of the body at 7th day after transplantation.Conclusion UCMSCs can aggregate and proliferate in damaged ovarian organs of mice through local ovarian injection.The local ovarian injection may achieve better therapeutic effect than the tail vein injection.

关 键 词：脐带间充质干细胞 移植 卵巢 活体成像 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]