应用同位素标记相对和绝对定量技术分析早期骨折合并颅脑损伤患者血清蛋白组织化学差异变化  

Analysis of serum proteomics in patients with early fracture complicated with craniocerebral injury by isobaric tags for relative and absolute quantification technique

作　　者：王斌[1] 杨冰 童九辉 张华[1] 马维[1] Wang Bin;Yang Bing;Tong Jiuhui;Zhang Hua;Ma Wei(Department of Orthopedics,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院骨科,石家庄050000

出　　处：《中华实验外科杂志》2021年第5期935-938,共4页Chinese Journal of Experimental Surgery

摘　　要：目的利用同位素标记相对和绝对定量(iTRAQ)技术分析寻找单纯股骨骨折和股骨骨折合并颅脑损伤患者早期差异表达基因。方法收集2019年5月至2020年5月就诊于河北医科大学第二医院骨科诊断为颅脑损伤合并股骨骨折患者和单纯股骨骨折患者各5例,分别取其伤后第1天和第3天的血清,应用iTRAQ技术比较两组患者血清蛋白组学的差异,用T-test检验对蛋白质定量结果进行统计分析。结果两组两个时间点均上调差异表达基因涉及的KEGG通路包括蛋白酶体、Wnt信号通路和HIF-1信号通路(P<0.05)。根据PPI可视化网络互作分析,各差异基因在第1天和第3天均上调且密切交互的基因依次为蛋白酶体亚单位α2型(PSMA2)第1天实验组高于对照组[(236.40±15.08)比(77.56±3.86),F=0.316,P<0.05],第3天实验组高于对照组[(376.20±10.69)比(80.14±6.56),F=1.191,P<0.05],差异有统计学意义、纤溶酶原激活物抑制物1型(SERPINE1)第1天实验组高于对照组[(107.14±7.47)比(49.50±4.53),F=1.566,P<0.05],第3天实验组高于对照组[(78.74±8.67)比(33.40±3.85),F=4.605,P<0.05]、原肌球蛋白4(TPM4)第1天实验组高于对照组[(207.40±11.73)比(80.60±6.27),F=0.010,P<0.05],差异有统计学意义,第3天实验组高于对照组[(4230.18±182.35)比(1325.66±96.43),F=2.057,P<0.05],差异有统计学意义、胰岛素样生长因子(IGF-1)第1天实验组高于对照组[(4974.81±163.97)比(978.82±110.92),F=0.343,P<0.05],差异有统计学意义,第3天实验组高于对照组[(907.46±95.98)比(355.51±21.95),F=4.240,P<0.05],差异有统计学意义、蛋白酶体亚单位α-4型(PSMA4)第1天实验组高于对照组[(1276.51±128.59)比(586.86±72.51),F=2.981,P<0.05],差异有统计学意义,第3天实验组高于对照组[(1648.67±107.21)比(541.43±55.93),F=1.559,P<0.05],差异有统计学意义,和肌球蛋白调节轻链10(MYL10)第1天实验组高于对照组[(118.84±12.78)比(51.43±5.48),F=4.139,P<0.05],差异有统计学意义,Objective To search for early differentially expressed genes in patients with femoral fracture alone and femoral fracture combined with craniocerebral injury by isotope labelling relative and absolute quantitative(iTRAQ)technique.Methods In May 2019-May 2020 in clinic in the second hospital,hebei medical university during the diagnosis of traumatic brain injury patients with femoral fractures and simple femoral fracture patients,5 cases were taken its serum 1 and 3 days after injury,using iTRAQ technology analysis comparing the difference between the two groups of patients with serum proteomics,using T-test for protein quantitative test results were analyzed.Results KEGG pathways involved in up-regulation of differentially expressed genes at two time points in both groups included proteasome,Wnt signaling pathway and HIF-1 signaling pathway(P<0.05).According to PPI visual network interaction analysis,the genes that were up-regulated and closely interacting on the first and third day were proteasome subunitα2(PSMA2)in the experimental group was higher than that in the control group on the first day[(236.40±15.08)vs.(77.56±3.86),F=0.316,P<0.05].On day 3,the experimental group was higher than the control group[(376.20±10.69)vs.(80.14±6.56),F=1.191,P<0.05].Plasminogen activator inhibitor type 1(SERPINE1)on day 1 was higher in the experimental group than the control group[(107.14±7.47)vs.(49.5±4.53),F=1.566,P<0.05],the experimental group was higher than the control group on day 3[(78.74±8.67)vs.(33.40±3.85),F=4.605,P<0.05],tropomyosin 4(TPM4)in the experimental group was higher than that in the control group on day 1[(207.40±11.73)vs.(80.60±6.27),F=0.010,P<0.05],On day 3,the experimental group was higher than the control group[(4230.18±182.35)vs.(1325.66±96.43),F=2.057,P<0.05],the experimental group was higher than the control group on day 1[(4974.81±163.97)vs.(978.82±110.92),F=0.343,P<0.05],on the third day,the experimental group was higher than the control group[(907.46±95.98)vs.(355.51±21.95),F=4.2

关 键 词：骨折 颅脑外伤 同位素标记 

分 类 号：R683.4[医药卫生—骨科学] R651.15[医药卫生—外科学]