缺氧及缺氧再复氧对正常骨组织发生与发展的影响  

Effects of hypoxia and hypoxia reoxygenation on the development of normal bone tissue

作　　者：王欢 董川 李彬 王伟 王璞 马琼 文艳华 刘云燕 马保安 WANG Huan;DONG Chuan;LI Bin;WANG Wei;WANG Pu;MA Qiong;WEN Yanhua;LIU Yunyan;MA Baoan(Department of Orthopedics,Tangdu Hospital of Air Force Medical University,Xi’an 710038,China)

机构地区：[1]空军军医大学唐都医院骨科,陕西西安710038 [2]解放军联勤保障部队第941医院,青海西宁810007

出　　处：《陕西医学杂志》2021年第6期664-668,共5页Shaanxi Medical Journal

摘　　要：目的:观察缺氧及缺氧再复氧对小鼠正常骨组织发生与发展的影响。方法:将健康的雄性C57小鼠40只随机分为常氧组、低氧组及低氧转常氧组。常氧组和低氧组分别饲养于常氧和低氧环境8周,而低氧转常氧组于低氧环境饲养4周后转至常氧环境饲养4周。于4周和8周处死小鼠,采用micro-CT、三点弯曲试验及HE染色观察小鼠股骨微观结构及功能变化。结果:4周时,低氧组骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)均低于常氧组(均P<0.05),骨表面积和组织体积比(BS/TV)高于常氧组(P<0.05);三维重建及HE染色观察低氧组骨小梁出现变短、断裂、网状结构退化,空隙较大,而常氧组骨小梁较粗,空隙较小,数量较多,连续性较好;生物力学检测显示低氧组小鼠股骨最大力、刚度、最大应力及杨氏模量均低于常氧组(均P<0.05)。8周时,低氧组BV/TV、Tb.Th、Tb.N均低于常氧组和低氧转常氧组,BS/TV高于常氧组与低氧转常氧组(均P<0.05),而常氧组与低氧转常氧组比较均无统计学差异(均P>0.05);三维重建及HE染色显示,低氧组骨小梁断裂、退化明显,空隙明显,而低氧转常氧组骨小梁的空隙小且均匀,连续性较好;生物力学检测显示低氧转常氧组小鼠股骨最大力、刚度、最大应力及杨氏模量与常氧组比较均无统计学差异(均P>0.05),但均高于低氧组(均P<0.05)。结论:低氧环境会造成骨质流失。在此情况下,复氧对于恢复骨组织结构和功能有积极作用,提示复氧可改善缺氧对骨组织结构和功能的影响。Objective:To observe the effects of hypoxia and hypoxia reoxygenation on the development of normal bone tissue in mice.Methods:40 healthy male C57 mice were randomly divided into normoxia group,hypoxia group,and hypoxia-to-normoxia group.The normoxia group and hypoxia group were fed in normoxic and hypoxic environment for 8 weeks,respectively,while the hypoxia-to-normoxia group was fed in hypoxic environment for 4 weeks and then turned to normoxic environment for 4 weeks.The mice were sacrificed at 4 and 8 weeks to observe the changes of femur microstructure and function by micro-CT,three-point bending test and HE staining.Results:At 4 weeks,BV/TV,Tb.Th and Tb.N in the hypoxia group were lower than those in the normoxia group(all P<0.05);BS/TV was higher than that of the normoxia group(P<0.05);three-dimensional reconstruction and HE staining observed that the trabecula of the hypoxia group was shortened,fractured,and the mesh structure was degenerated,and the gap was larger,while the trabecula of the normoxia group was thicker,with smaller voids,more number,and better continuity;biomechanical testing showed that the maximum force,stiffness,maximum stress and Young’s modulus of the femur of the hypoxia group were lower than those of the normoxia group(All P<0.05).At 8 weeks,the BV/TV,Tb.Th,Tb.N of the hypoxia group were lower than those of the normoxia group and the hypoxia-to-normoxia group,and BS/TV was higher than the normoxia group and the hypoxia-to-normoxia group(all P<0.05),while there was no statistical difference between the normoxia group and the hypoxia-to-normoxia group(all P>0.05);three-dimensional reconstruction and HE staining showed that the hypoxia group had obvious fracture and degeneration of bone trabeculae,obvious voids,while the of the hypoxia-to-normoxia group had small and uniform trabecular bone gap and good continuity;biomechanical testing showed that the maximum force,stiffness,maximum stress and Young’s modulus of the femur in the hypoxia-to-normoxia group were not statistically dif

关 键 词：缺氧 再复氧 骨组织 三维重建 生物力学 骨质疏松 

分 类 号：Q954.658[生物学—动物学]