青蒿琥酯对2型糖尿病大鼠下颌下腺IL-1β表达的影响  

The effect of artesunate on the expression of IL-1βin the submandibular gland of type 2 dia⁃betic rats

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作  者:李佳芮 张思琴 农晓琳[1] Li Jiarui;Zhang Siqin;Nong Xiaolin(College&Hospital of Stomatology,Guangxi Medical University,Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Guangxi Clinical Research Center for Craniofacial Deformity,Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment,Nanning 530021,China)

机构地区:[1]广西医科大学口腔医学院/附属口腔医院广西口腔颌面修复与重建研究自治区级重点实验室广西颅颌面畸形临床医学研究中心颌面外科疾病诊治研究重点实验室广西高校重点实验室,南宁530021

出  处:《广西医科大学学报》2021年第5期980-984,共5页Journal of Guangxi Medical University

基  金:广西自然科学基金重点项目(No.2019GXNSFDA245016)。

摘  要:目的:研究青蒿琥酯(ART)对2型糖尿病(T2DM)大鼠下颌下腺中IL-1β表达的影响。方法:将24只健康雄性SD大鼠随机分为正常对照组、模型组和ART组。模型组和ART组给予高糖高脂饲料喂养,8周后腹腔注射35 mg/kg链脲佐菌素(STZ)复制T2DM模型。用药干预4周后,观察各组大鼠体重、空腹血糖、唾液流量的变化,分别采用实时荧光定量PCR(qPCR)法和免疫组化法检测大鼠下颌下腺中IL-1β基因和蛋白的表达。结果:与模型组相比,ART组大鼠血糖显著降低,体重显著增加,唾液流量明显增加(P<0.05)。模型组IL-1βmRNA及蛋白表达较正常对照组显著上调,ART组大鼠IL-1βmRNA及蛋白表达较模型组显著下调(P<0.05)。结论:ART能降低T2DM大鼠血糖,增加其唾液流量,减轻下颌下腺损伤,其机制可能与抑制下颌下腺中IL-1β的表达有关。Objective:To investigate the effect of artesunate(ART)on the expression of IL-1βin the submandibular gland of type 2 diabetic(T2DM)rats.Methods:Twenty-four healthy male SD rats were randomly divided into control group,model group and ART group.Model group and ART group rats were fed with high-sugar and highfat diet,and were intraperitoneally injected with 35mg/kg streptozotocin(STZ)8 weeks later to replicate the T2DM model.After 4 weeks of drug intervention,the changes of body weight,fasting blood glucose and salivary flow in each group were observed.The expression of IL-1βin submandibular gland was detected by real-time qPCR and immunohistochemical method.Results:Compared with model group,the blood glucose was significantly decreased while the body weight and salivary flow were notably increased in ART group(P<0.05).Compared with the control group,the mRNA and protein expression of IL-1βin the model group wasevidently up-regulated,and that was significantly down-regulated in the ART group compared with the model group(P<0.05).Conclusion:ART can reduce blood glucose,increase salivary flow and alleviate submandibular gland injury in T2DM rats.The mechanism may be related to the inhibition of IL-1βexpression in the submandibular glands.

关 键 词:2型糖尿病 下颌下腺 青蒿琥酯 IL-1Β 

分 类 号:R963[医药卫生—微生物与生化药学]

 

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