白花蛇舌草上调miR-485-5p抑制人膀胱癌细胞系KU-19-19增殖、迁移和侵袭  

Hedyotis diffusa inhibits the proliferation,migration and invasion of bladder cancer cell line KU-19-19 through up-regulating miR-485-5p

作　　者：徐林飞[1] 张海涛[1] 刘晟[1] 蔡海荣[1] 胡恩平[1] XU Lin-fei;ZHANG Hai-tao;LIU Sheng;CAI Hai-rong;HU En-ping(Department of Urology,Taizhou Municipal Hospital,Taizhou 318000,China)

机构地区：[1]台州市立医院泌尿外科,浙江台州318000

出　　处：《基础医学与临床》2021年第6期842-847,共6页Basic and Clinical Medicine

基　　金：浙江省自然科学基金(Y12H050048);2020年台州农业科技计划项目和第一批社会发展科技计划(20ywa33)。

摘　　要：目的探讨白花蛇舌草对膀胱癌细胞系KU-19-19增殖、迁移和侵袭的影响和可能的分子机制。方法将KU-19-19细胞分为对照组、白花蛇舌草组、miR-NC组、miR-485-5p组、anti-miR-NC组、anti-miR-485-5p组、白花蛇舌草+anti-miR-NC组、白花蛇舌草+anti-miR-485-5p组、白花蛇舌草+anti-miR-485-5p+LY294002组。细胞计数试剂盒(CCK-8)、Transwell实验、实时荧光定量PCR(RT-qPCR)分别检测细胞增殖、迁移侵袭以及miR-485-5p的表达。蛋白质印迹(Western blot)检测磷脂酰肌醇-3-羟激酶(PI3K)和蛋白激酶B(AKT)的磷酸化水平。结果白花蛇舌草干预后KU-19-19细胞增殖率、迁移侵袭细胞数显著降低,miR-485-5p表达显著升高(P<0.05);过表达miR-485-5p后,KU-19-19细胞增殖率、迁移侵袭细胞数显著降低(P<0.05);抑制miR-485-5p表达后,KU-19-19细胞增殖率、迁移侵袭细胞数显著升高(P<0.05)。抑制miR-485-5p可以逆转降低白花蛇舌草对KU-19-19细胞增殖、迁移和侵袭的抑制作用(P<0.05);LY294002可以逆转降低抑制miR-485-5p对白花蛇舌草处理的KU-19-19细胞增殖、迁移和侵袭的促进作用(P<0.05)。结论白花蛇舌草通过上调miR-485-5p可抑制膀胱癌细胞的增殖、迁移和侵袭,其机制可能与抑制PI3K/AKT信号通路有关。Objective To explore the effect and possible molecular mechanism of Hedyotis diffusa on the proliferation,migration and invasion of bladder cancer cells.Methods U-19-19 cells were divided into control group,Hedyotis diffusa group,miR-NC group,miR-485-5p group,anti-miR-NC group,anti-miR-485-5p group,Hedyotis diffusa+anti-miR-NC group,Hedyotis diffusa+anti-miR-485-5p group,Hedyotis diffusa+anti-miR-485-5p+LY294002 group.Cell counting kit(CCK-8),Transwell experiment,and real-time fluorescent quantitative PCR(RT-qPCR)were used to detect cell proliferation,migration,invasion and miR-485-5p expression.Western blot was performed to detect the phosphorylation levels of phosphatidylinositol-3-hydroxykinase(PI3K)and protein kinase B(AKT).Results After the intervention of Hedyotis diffusa,the proliferation rate,and the cell numbers of migrat and invade of KU-19-19 cells were significantly reduced,and the expression of miR-485-5p was significantly increased(P<0.05).After overexpression of miR-485-5p,the proliferation rate,and the cell numbers of migrat and invade of KU-19-19 cells were significantly reduced(P<0.05).After inhibiting the expression of miR-485-5p,the proliferation rate,and the cell numbers of migrat and invade of KU-19-19 cells were significantly increased(P<0.05).Inhibition of miR-485-5p could reverse the inhibitory effect of Hedyotis diffusa on KU-19-19 cell proliferation,migration and invasion(P<0.05).LY294002 could reverse the promoted effect of inhibiting miR-485-5p on proliferation,migration and invasion of KU-19-19 cells treated with Hedyotis diffusa(P<0.05).Conclusions Hedyotis diffusa can inhibit the proliferation,migration and invasion of bladder cancer cells by up-regulating miR-485-5p.The mechanism may be related to the inhibition of PI3K/AKT signaling pathway.

关 键 词：白花蛇舌草 miR-485-5p 膀胱癌 增殖 迁移和侵袭 PI3K/AKT信号通路 

分 类 号：R286.91[医药卫生—中药学]