象甲科昆虫转录组研究进展  

Superficial analysis of the transcriptome in Curculionidae

作　　者：杨红军 徐丹萍 胡佳萌 卓志航 陈彧 YANG Hongjun;XU Danping;HU Jiameng;ZHUO Zhihang;CHEN Yu(College of Life Science,China West Normal University,Nanchong,Sichuan 637002,China;College of Forestry,Hainan University,Haikou,Hainan 570228,China;Key Laboratory of Integrated Pest Management on Crops in South China,Ministry of Agriculture,P.R.China,Guangzhou,Guangdong 510642,China;Hainan Academy of Forestry(Hainan Academy of Mangrove),Haikou,Hainan 571100,China)

机构地区：[1]西华师范大学生命科学学院,四川南充637002 [2]海南大学林学院,海南海口570228 [3]农业部华南作物有害生物综合治理重点实验室,广东广州510642 [4]海南省林业科学研究院(海南省红树林研究院),海南海口571100

出　　处：《生物安全学报》2021年第2期89-96,共8页Journal of biosafety

基　　金：海南省自然科学基金(319QN163);农业部华南作物有害生物综合治理重点实验室2017/2018年度开放基金(SCIPM2018-04);海南大学科研启动项目[KYQD(ZR)1823];四川省第二批地方普通本科高校应用型示范专业(川教函〔2019〕31号)。

摘　　要：转录组是细胞在特定发育阶段或生理条件下的全部转录本及其数量总和,并能揭示特定状态下的基因表达模式及分子机理。象甲科昆虫拥有动物界中最多的物种种类,该科许多昆虫也是我国粮食仓储和农林产业上的重大害虫。通过研究象甲科昆虫转录组,从分子水平揭示其生命过程中相关的基因及其功能,对寻找害虫防治的新方法具有重要意义。本文对象甲科昆虫转录组研究现状进行了浅析,涉及象甲科不同发育阶段基因差异、昆虫触角、与植物互作、防治、免疫机制、进化、基因沉默7个方面的分析,可在理论上丰富对象甲科昆虫遗传发育、免疫、潜在RNA干扰靶点筛选等方面的理解,并在此基础上提出了象甲科转录组今后的研究热点与应用前景,为害虫防控提供理论指导。Analysis of the transcriptome,which is a snapshot of the total transcription in a cell at a particular developmental stage or condition,can reveal the gene expression patterns and molecular mechanisms in a specific state.Curculionidae is the largest family in the kingdom Animalia,and many species of Curculionidae are pests that affect grain storage,agriculture,and forestry in China.To determine the gene functions related to life processes,to search for novel pest control targets,and above all,to understand the genetics of insect development and immunity and screen potential RNA interference(RNAi)targets,it is important to study the transcriptome of Curculionidae.In this work,the transcriptome of Curculionidae was superficially analyzed,and the differentially expressed genes at different developmental stages,in insect antennae,during interaction with plants,and related to prevention and control,immune mechanisms,evolution,and RNAi were identified.In addition,the prospects for in-depth research of Curculionidae insects and potential applications are discussed,and theoretical guidance for pest control is provided.

关 键 词：象甲科 转录组 研究概况 基础应用 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]