玉米转录因子基因ZmMYB308的克隆及表达分析  

Cloning and Expression Analysis of Transcription Factor Gene ZmMYB 308 in Maize

作　　者：王新涛 杨青 代资举 李保叶 郝俊杰[1] WANG Xintao;YANG Qing;DAI Ziju;LI Baoye;HAO Junjie(Plant Protection Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Henan Academy of Crop Molecular Breeding,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南省农业科学院芝麻研究中心,郑州450002 [3]河南省作物分子育种研究院,郑州450002

出　　处：《西北植物学报》2021年第5期738-745,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：河南省科技攻关计划(192102110026,202102110155);河南省农业科学院科研发展专项(2020CY011,2020YQ33)。

摘　　要：MYB类转录因子广泛地参与了植物生长发育的许多重要过程,在调控木质素合成途径中也起着重要作用。为探索MYB转录因子在玉米发育中的功能,该研究利用玉米茎秆转录组测序数据,对玉米茎秆发育过程中差异表达的MYB转录因子进行筛选和分析,在此基础上,通过RT-PCR方法克隆获得了ZmMYB 308基因。结果表明:(1)共检测到14个差异表达的MYB转录因子,其中10个下调表达,4个上调表达。(2)ZmMYB 308基因包含一个747 bp的开放阅读框,可编码248个氨基酸,相对分子质量27.01 kD,等电点为9.17;系统进化树比对表明,玉米ZmMYB308蛋白和谷子SiMYB308蛋白的亲缘关系较近,相似性高达94%。(3)实时荧光定量PCR结果显示,ZmMYB 308在玉米不同发育时期和不同组织中的表达差异显著,且ZmMYB 308基因的表达随着玉米茎秆发育的推进呈先升高后降低的趋势,在吐丝期表达量达到最高,随后在灌浆期显著下降;不同组织定量分析显示,ZmMYB 308基因在木质素含量高的茎秆和根中高表达。研究推测,ZmMYB 308基因可能在玉米茎秆发育过程中起着重要作用,该研究结果为进一步探索玉米木质素合成的分子机制奠定了基础。MYB transcription factors are widely involved in plant growth and development,and also play an important role in regulating lignin synthesis.In this study,in order to explore the role of MYB transcription factor in maize development,the differentially expressed MYB transcription factor genes were screened and analyzed based on the transcriptome sequencing data of maize stems,and the ZmMYB 308 gene was cloned by RT-PCR method.The results showed that:(1)a total of 14 differentially expressed MYB transcription factor genes were detected in different periods,among which 10 were down-regulated and 4 were up-regulated.(2)ZmMYB 308 contains a 747 bp open reading frame,which encodes a 248 amino acids polypeptide with a protein molecular weight of about 27.01 kD and a theoretical isoelectric point of 9.17.Blast results showed that ZmMYB308 was closely related to SiMYB308 of foxtail millet with 94%similarity.(3)The results of real time quantitative PCR analysis showed that ZmMYB 308 was significantly differentially expressed in different development periods and tissues.The expression of the ZmMYB 308 first increased gradually and then decreased with the development of maize stem,and reached the peak at the silking stage.Meanwhile,Zm MYB 308 was highly expressed in maize stems and roots which had lignin content.The results indicated that ZmMYB 308 might play an important role in the regulation of stem growth and development.This work lays the foundation for further exploring the molecular regulatory mechanism of lignin synthesis in maize.

关 键 词：玉米 MYB转录因子 ZmMYB 308 荧光定量PCR 

分 类 号：Q785[生物学—分子生物学] Q786