‘中山杉302’×墨西哥落羽杉回交子代叶片光合性状和叶绿素含量的QTL定位  

QTL mapping of photosynthetic traits and chlorophyll content in leaf of backcross progenies of Taxodium ‘Zhongshanshan 302’× T. mucronatum

作　　者：郭金博 华建峰[1] 殷云龙[1] 杨颖 GUO Jinbo;HUA Jianfeng;YIN Yunlong;YANG Ying(Jiangsu Engineering Research Center for Taxodium Rich.Germplasm Innovation and Propagation,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)江苏省落羽杉属树木种质创新与繁育工程研究中心,江苏南京210014 [2]南京林业大学生物与环境学院,江苏南京210037

出　　处：《植物资源与环境学报》2021年第3期1-7,共7页Journal of Plant Resources and Environment

基　　金：国家自然科学基金资助项目(31700588);江苏省政策引导类计划(引进外国人才专项)资金项目(BX2020010);中央财政林业科技推广示范资金项目(苏[2020]TG04)。

摘　　要：对‘中山杉302’(Taxodium‘Zhongshanshan 302’)×墨西哥落羽杉(T.mucronatum Tenore)回交子代叶片的光合性状(包括净光合速率、气孔导度、胞间CO_(2)浓度和蒸腾速率)及叶绿素含量(包括叶绿素a、叶绿素b和总叶绿素含量)进行统计分析,并对这些性状进行QTL定位。结果表明:这7个性状的频率呈正态分布。净光合速率均值为3.91μmol·m^(-2)·s^(-1),气孔导度均值为0.23 mmol·m^(-2)·s^(-1),胞间CO_(2)浓度均值为190.94μmol·mol-1,蒸腾速率均值为5.17 mml·m^(-2)·s^(-1),叶绿素a、叶绿素b和总叶绿素含量均值分别为0.48、0.16和0.64 mg·g^(-1);胞间CO_(2)浓度变异系数最小(7.39%),其余6个性状变异系数较大,均在25.00%及以上,以气孔导度变异系数最大(43.48%)。QTL定位结果表明:除蒸腾速率和叶绿素b含量外,其余5个性状共定位到8个主效QTLs,包含98个SLAF标记,且这些主效QTLs的置信区间不同。净光合速率、气孔导度和叶绿素a含量各定位到1个主效QTL,分别位于1号连锁群(q 1-1)和11号连锁群(q 11-1和q 11-2),各包含25、3和13个SLAF标记,解释表型贡献率分别为5.68%、7.85%和10.95%;胞间CO_(2)浓度定位到2个主效QTLs,均位于6号连锁群(q 6-1和q 6-2),各含3和1个SLAF标记,解释表型贡献率分别为9.76%~10.70%和0.62%;总叶绿素含量定位到3个主效QTLs,分别位于1号连锁群(q 1-2)和11号连锁群(q 11-3和q 11-4),各包含26、26和1个SLAF标记,解释表型变异率为6.51%~6.64%、9.66%和6.60%。并且,q 6-2的加性效应值为正值,其余QTLs的加性效应值均为负值。研究结果显示:这8个主效QTLs的置信区间不同,不存在“一因多效”现象,且这些QTL区间基因主要参与光合性状和叶绿素含量的负调控。Statistical analysis was conducted for photosynthetic traits(including net photosynthetic rate,stomatal conductance,intercellular CO_(2)concentration,and transpiration rate)and chlorophyll content(including chlorophyll a,chlorophyll b,and total chlorophyll contents)in leaf of backcross progenies of Taxodium‘Zhongshanshan 302’×T.mucronatum Tenore,and QTL mapping was performed for these traits.The results show that the frequency of these 7 traits shows a normal distribution.The mean of net photosynthetic rate is 3.91μmol·m^(-2)·s^(-1),that of stomatal conductance is 0.23 mmol·m^(-2)·s^(-1),that of intercellular CO_(2)concentration is 190.94μmol·mol-1,that of transpiration rate is 5.17 mmol·m^(-2)·s^(-1),and those of chlorophyll a,chlorophyll b,and total chlorophyll contents are 0.48,0.16,and 0.64 mg·g-1,respectively;the coefficient of variation of intercellular CO_(2)concentration is the smallest(7.39%),while those of other 6 traits are relatively large,all of them are 25.00%and above,and the coefficient of variation of stomatal conductance is the largest(43.48%).The QTL mapping result shows that except for transpiration rate and chlorophyll b content,other 5 traits are mapped to 8 major QTLs,which contain 98 SLAF markers,and the confidence intervals of these major QTLs are different.Net photosynthetic rate,stomatal conductance,and chlorophyll a content are each mapped to 1 major QTL,which locate at No.1 linkage group(q 1-1)and No.11 linkage group(q 11-1 and q 11-2),each contains 25,3,and 13 SLAF markers,respectively,and their rates of explaining phenotypic variation are 5.68%,7.85%,and 10.95%,respectively;intercellular CO_(2)concentration is mapped to 2 major QTLs,which locate at No.6 linkage group(q 6-1 and q 6-2),each contains 3 and 1 SLAF markers,respectively,and their rates of explaining phenotypic variation are 9.76%-10.70%and 0.62%,respectively;total chlorophyll content is mapped to 3 major QTLs,which locate at No.1 linkage group(q 1-2)and No.11 linkage group(q 11-3 and q 11-4),each contains 2

关 键 词：落羽杉属 ‘中山杉’ 光合性状 叶绿素 QTL定位 SLAF标记 

分 类 号：Q943.2[生物学—植物学] S791[农业科学—林木遗传育种]