植冰技术在低温生物医学中的应用进展  

Application Progress of Ice Seeding Technology in Low Temperature Biomedicine

作　　者：李维杰[1] 宋立勇 刘宝林[1] LI Weijie;SONG Liyong;LIU Baolin(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《制冷技术》2021年第2期11-16,共6页Chinese Journal of Refrigeration Technology

基　　金：上海市上海理工大学医工交叉(No.1020308412);上海市科委(No.18441909700);上海地方能力建设(No.19060502400)。

摘　　要：植冰技术由于能够有效低温保存细胞而被广泛采用。本文综述了植冰技术的发展历程,包括通过接触外壁、添加冰成核剂、电极诱导、超声波诱导、改变降温速率以及改变压力诱导成核等技术。结果表明:通过以上6种诱导成核的方式,能够诱导溶液在较高的零下温度成核,降低过冷度,改变冰晶的形态,降低胞内冰损伤。但是添加冰成核剂、超声波诱导、改变降温速率以及改变压力诱导等方法有一个缺点,无法控制在特定温度下促进成核,这不利于探究植冰温度对细胞存活率的影响。Ice seeding technology has been widely adopted,because it can effectively preserve cells at low temperature.In this paper the history of ice seeding and the methods of ice seeding are introduced.The methods induced nucleation include touching outer wall,adding ice nucleating agent,electrode induction,ultrasound induction,changing cooling rate and changing pressure.The results show that the above six ways of inducing nucleation can induce the solution to promote nucleation at a higher sub-zero temperature,reduce the degree of supercooling,change the shape of ice crystals,and reduce intracellular ice damage.However,adding ice nucleating agents,ultrasonic induction,changing the cooling rate,and changing the pressure induction methods have a disadvantage that they cannot be controlled to promote nucleation at a specific temperature,which is not conducive to explore the effect of ice seeding temperature on cell viability.

关 键 词：植冰技术 低温保存 过冷度 胞内冰 

分 类 号：TB611[一般工业技术—制冷工程] R318.52[医药卫生—生物医学工程]