滋阴化痰方通过调控外泌体对小鼠胃癌的干预作用  

The interventitive effect of Ziyin-Huatan Decoction by regulating exosomes on mice with gastric cancer

作　　者：李晶晶 季青[2] 刘煊[1] 李琦[2] 岳小强[1] Li Jingjing;Ji Qing;Liu Xuan;Li Qi;Yue Xiaoqiang(Department of Traditional Chinese Medicine,Changzheng Hospital Affiliated to the Second Military Medical University,Shanghai 200003,China;Cancer Institute of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200003,China)

机构地区：[1]第二军医大学长征医院中医科,上海200003 [2]上海中医药大学附属曙光医院肿瘤研究所,200003

出　　处：《国际中医中药杂志》2021年第5期472-476,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81603434、81804008)。

摘　　要：目的观察滋阴化痰方调控外泌体对小鼠胃癌皮下瘤的干预作用。方法取对数生长期的第4代人胃癌细胞株MGC-803细胞,按随机数字表法分为外泌体对照组和低、高剂量组,低、高剂量组分别加入25、100μg/ml的滋阴化痰方进行干预,48 h后提取各组MGC-803细胞分泌的外泌体。将20只BALB/c雄性小鼠按随机数字表法分为外泌体对照组、滋阴化痰方低剂量组、滋阴化痰方高剂量组和空白对照组,每组5只。除空白对照组外,其余各组小鼠分别经眼眶注射对应组细胞提取的外泌体,每只10μg/次,隔日1次,共15次;空白对照组注射等量PBS。末次给药后以SGC-7901细胞接种小鼠建立肿瘤模型。观测小鼠体重及成瘤情况。采用免疫组化染色观察肿瘤组织血小板-内皮细胞黏附分子(platelet endothelial cell adhesion molecule-1,CD31)、VEGF、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFG)表达。结果与空白对照组比较,滋阴化痰方高剂量组肿瘤重量[(170.00±10.00)mg比(343.33±20.82)mg]降低(P<0.05),肿瘤CD31[(37.43±0.55)比(63.30±0.85)]、VEGF[(11.37±1.19)比(70.30±0.72)]、bFGF[(43.77±1.53)比(84.97±1.86)]表达均降低(P<0.05)。与外泌体对照组比较,滋阴化痰方低、高剂量组CD31、VEGF、bFGF表达均降低(P<0.05)。结论滋阴化痰方可调控外泌体抑制小鼠胃癌皮下瘤的生长,这种作用可能与抑制肿瘤血管新生有关。Objective To observe the interventative effect of Ziyin-Huatan Decoction by regulating exosomes on subcutaneous tumor of mice with gastric cancer.Methods MGC-803 cells were randomly divided into exosome control group,low-dose group and high-dose group.The low-dose group and high-dose group were intervened with Ziyin-Huatan Decoction of 25μg/ml and 100μg/ml respectively.After 48 hours,the exosomes secreted by MGC-803 cells in each group were extracted.Twenty BALB/c male mice were randomly divided into exosome control group,low-dose Ziyin-Huatan Decoction group,high-dose Ziyin-Huatan Decoction group and blank control group,with 5 mice in each group.Except for the blank control group,the mice in the other groups were injected with exosomes extracted from the cells of the corresponding group through the orbit,10μg/time for each mouse,once every other day,a total of 15 times;the blank control group was injected with the same amount of PBS.Then SGC-7901 cells were inoculated into mice to establish a tumor model.The tumorigenic rate and body weight of mice were observed.The levels of CD31,VEGF and bFGF in tumor tissues were detected by immunohistochemistry.Results Compared with the blank control group,the tumor weight[(170.00±10.00)mg vs.(343.33±20.82)mg]and the expression of CD31(37.43±0.55 vs.63.30±0.85),VEGF(11.37±1.19 vs.70.30±0.72)and bFGF(43.77±1.53 vs.84.97±1.86)in the high-dose Ziyin-Huatan Decoction group significantly decreased(P<0.05).Compared with exosome control group,the expressions of CD31,VEGF and bFGF in low and high dose Ziyin-Huatan Decoction groups were decreased(P<0.05).Conclusion Ziyin-Huatan Decoction can significantly inhibit the growth of subcutaneous tumor of gastric cancer in mice by regulating exudation,which may be related to the inhibition of tumor angiogenesis.

关 键 词：外泌体 胃癌 血管新生 滋阴化痰方 小鼠 

分 类 号：R285.5[医药卫生—中药学]