2451份不同类型标本中人巨细胞病毒DNA的检出情况分析  

The analysis on the detection of human cytomegalovirus DNA in 2451 samples of different types

作　　者：刘孟涓 李秋红[1] 李春莉[1] LIU Mengjuan;LI Qiuhong;LI Chunli(Department of Clinical Laboratory,Chongqing Health Center for Women and Children,Chongqing 401147,China)

机构地区：[1]重庆市妇幼保健院检验科,重庆401147

出　　处：《检验医学与临床》2021年第11期1563-1566,1570,共5页Laboratory Medicine and Clinic

摘　　要：目的对孕妇和婴儿不同类型标本中人巨细胞病毒(HCMV)DNA的检出情况进行回顾性分析,为尽早有效地检出HCMV DNA提供参考依据。方法收集2017年5月至2020年7月在该院检验科采用荧光定量PCR法进行HCMV DNA检测的临床标本共2451份,对不同标本来源人群、不同类型标本的HCMV DNA检出率进行回顾性分析。结果孕妇的HCMV DNA的总体检出率为3.80%,<35岁和≥35岁两个年龄段孕妇的检出率比较,差异无统计学意义(P>0.05)。0~6个月婴儿的总体检出率为9.63%;不同性别婴儿的检出率比较差异无统计学意义(P>0.05);除母亲乳汁外,全血、血清、尿液标本的检出率在新生儿(<28 d)和28 d至6个月婴儿间差异均有统计学意义(P<0.05)。不同类型的标本中,乳汁的检出率最高(78.49%),其次为脐血(20.00%)和尿液(6.82%),而羊水检出率最低(1.17%),脑脊液标本仅有1份且未检出。若同一婴儿采集多种类型的标本,母亲乳汁标本的检出率明显高于尿液和血清,差异有统计学意义(P<0.05)。若同一孕妇中采集多种类型的标本,乳汁标本的检出率也高于其他类型标本(P<0.05)。结论孕妇和婴儿各年龄组的各类标本中,母亲乳汁标本的HCMV DNA检出率均明显高于其他类型的标本。因此对于血、尿HCMV DNA阴性而又高度疑似HCMV感染的孕妇或婴儿,建议采集乳汁标本进行检测,这有助于临床早期对HCMV DNA的检出。Objective To retrospectively analyse the detection of HCMV DNA in samples of different types from pregnant women and infants,and provide a reference for the effective detection of HCMV DNA in the early clinical stage.Methods A total of 2451 clinical specimens were collected from May 2017 to July 2020 in the laboratory of the hospital using fluorescence quantitative PCR for HCMV DNA detection.The HCMV DNA detection rates of different specimen sources and different types of specimens were retrospectively reviewed snd analyzed.Results The overall detection rate of HCMV DNA in pregnant women was 3.80%.There was no statistically significant difference in the detection rates of pregnant women between the ages of<35 and≥35(P>0.05).The overall detection rate of infants from 0 to 6 months was 9.63%;there was no significant difference in the detection rate of infants of different genders(P>0.05);except for mother′s milk,there was a statistically significant difference in the detection of whole blood,serum and urine specimens between newborns(<28 d)and 28 d to 6 month-old babies(P<0.05).Among different types of specimens,breast milk had the highest detection rate(78.49%),followed by cord blood(20.00%)and urine(6.82%),and amniotic fluid had the lowest detection rate(1.17%).There was only 1 cerebrospinal fluid specimen,in which HCMV DNA was not detected.If multiple types of specimens were collected from the same infant,the detection rate of maternal milk specimens was significantly higher than that of urine and serum,and the difference was statistically significant(P<0.05).If multiple types of specimens were collected from the same pregnant woman,the detection rate of breast milk specimens was also higher than that of other types of specimens(P<0.05).Conclusion Among the various specimens of pregnant women and infants of all age groups,the detection rate of HCMV DNA in maternal milk specimens was significantly higher than that of other types of specimens.Therefore,for pregnant women or babies with blood and urine HCMV DNA

关 键 词：人巨细胞病毒DNA 检出率 多种体液标本 婴儿 孕妇 

分 类 号：R446.5[医药卫生—诊断学]