Global transcriptomic analysis reveals Lnc-ADAMTS9 exerting an essential role in myogenesis through modulating the ERK signaling pathway  

作　　者：Liqi Wang Ting He Xin Zhang Yubo Wang Kai Qiu Ning Jiao Linjuan He Jingdong Yin 

机构地区：[1]State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,No.2 Yuanmingyuan West Road,Beijing 100193,China

出　　处：《Journal of Animal Science and Biotechnology》2021年第2期520-533,共14页畜牧与生物技术杂志（英文版）

基　　金：supported by the National key research and development program of China(Grant No.2018YFD0500402);the National Natural Science Foundation of China(Grant No.31790412,Grant No.31672431);the National Key Basic Research Program of China(2013CB127302。

摘　　要：Background:Long non-coding RNAs(lncRNAs)are emerging key regulators involved in a variety of biological processes such as cell differentiation and development.The balance between myogenesis and adipogenesis is crucial for skeletal muscle homeostasis in humans and meat quality in farm animals.The present study aimed to reveal the global transcriptomic profiles of adipogenic(Adi-)and myogenic(Myo-)precursors derived from porcine skeletal muscle and identify lncRNAs involved in the modulation of myogenesis homeostasis in porcine skeletal muscle.Results:In this study,a total of 655 novel individual lncRNAs including differentially expressed 24 lncRNAs,and 755 differentially expressed mRNAs were identified(fold change≥2 or≤0.5 and adjusted P<0.05).Integrated results of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis accompanied by the variation of intracellular Ca2+concentration highlighted Lnc-ADAMTS9 involved in the modulation of myogenesis homeostasis in porcine skeletal muscle.Although Lnc-ADAMTS9 knock-down did not alter the mRNA expression of ADAMTS9,we demonstrated that Lnc-ADAMTS9 can promote myogenic proliferation and myogenic differentiation of myogenic precursors through inhibiting the ERK/MAPK signaling pathway.Conclusion:We deciphered a comprehensive catalog of mRNAs and lncRNAs that might be involved in the regulation of myogenesis and adipogenesis homeostasis in the skeletal muscle of pigs.The Lnc-ADAMTS9 exerts an essential role in myogenesis through the ERK signaling pathway.

关 键 词：Adipogenic precursors LncRNA Myogenic differentiation Myogenic precursors Myogenic proliferation PIGS Skeletal muscle 

分 类 号：S852.2[农业科学—基础兽医学]