Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs  

作　　者：Junshi Shen Zhipeng Li Zhongtang Yu Weiyun Zhu 

机构地区：[1]Laboratory of Gastrointestinal Microbiology,Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]National Center for International Research on Animal Gut Nutrition,Nanjing Agricultural University,Nanjing 210095,China [3]Department of Special Animal Nutrition and Feed Science,Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China [4]Department of Animal Sciences,The Ohio State University,Columbus,OH 43210,USA

出　　处：《Journal of Animal Science and Biotechnology》2021年第2期677-688,共12页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(award No:31402101);the“One Belt and One Road”Technical Cooperation Project of Jiangsu Province(award No:BZ2018055);the Jiangsu Agriculture Science and Technology Innovation Fund(award No:CX(19)3023).

摘　　要：Background:Diet has a profound impact on the rumen microbiota,and the impact can vary among the different rumen ecological niches(REN).This study investigated the effects of dietary replacement of soybean meal(SBM)with dried distillers grains with solubles(DDGS)on the rumen microbiota occupying different REN of growing Hu lambs.After a 9-week feeding trial,6 lambs from each dietary treatment(SBM vs.DDGS-based diets)were slaughtered for sample collection.The microbiota of the rumen solid,liquid,and epithelium fractions was examined using amplicon sequencing analysis of bacterial 16S rRNA gene,functional prediction,and qPCR.Results:No interaction of dietary protein source(PS)and REN were detected for virtually all the measurements made in this study.The DDGS substitution resulted in very limited influence on bacterial community structure.However,the metabolic pathways predicted from 16S rRNA gene sequences varied greatly between SBM-and DDGS-based diets.The populations of rumen total bacteria,fungi,sulfate-reducing bacteria(SRB),and methanogens were not influenced by DDGS substitution,but the population of protozoa was reduced.The bacterial communities in rumen solid(RS)and liquid(RL)were similar in taxonomic composition but were different in relative abundance of some taxa.In contrast,the bacterial composition and relative abundance of rumen epithelium(RE)were greatly distinct from those of the RS and the RL.In alignment with the bacterial relative abundance,the metabolic pathways predicted from 16S rRNA genes also varied greatly among the different REN.The populations of total bacteria,protozoa,and methanogens attached to the RE were smaller than those in the RS and RL,and the fungal population on the rumen epitheliumwas smaller than that in the RS but similar to that in the RL.On the contrary,the SRB population on the RE was greater than that in the RS and RL.Conclusions:Substitution of SBM with DDGS had greater impact to the protozoa than to the other microbes,and the microbial community structure and functions

关 键 词：Distillers dried grains with solubles Growing lamb MICROBIOTA Ruminal ecological niche Soybean meal 

分 类 号：S826.5[农业科学—畜牧学]