液晶生物传感及其在微流控细胞分析中的应用  

Liquid Crystal Bio-sensing and Its Application in Microfluidic Cell Analysis

作　　者：李玮玮 李雨轩 林金明[1] LI Wei-wei;LI Yu-xuan;LIN Jin-ming(Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学化学系,微量分析测试方法和仪器研制北京市重点实验室生命有机磷化学和化学生物学教育部重点实验室,北京100084

出　　处：《分析测试学报》2021年第6期822-827,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(22034005,21621003)。

摘　　要：液晶(LC)生物传感器是基于LC对界面性质变化的高灵敏响应及其固有的光学各向异性发展起来的一种技术,在生物样品的检测分析方面展现出了非凡的应用价值。通过修饰刺激响应性分子,LC界面可以灵敏地响应待测生物分析物的存在,并诱导界面LC分子发生取向改变,而界面上LC分子的短程相互作用引起LC相本体分子的取向改变,在偏光显微镜(POM)下可见LC显示出不同的光学织构。因此,LC传感器无需对待测物进行标记,凭借其简单的光学检测仪器,容易将分子事件转化为可视化光学信号输出,具有简单、灵敏、高效、快速、廉价等优点,在生物检测分析领域得到了广泛应用。该文综述了近年来液晶生物传感器在检测分析生物小分子、生物大分子以及生物有机体等方面的研究进展,特别提到了液晶生物传感器引入微流控芯片用于细胞分析的新兴领域的发展与挑战。目前,液晶生物传感已经成功地应用于界面现象复杂多样的细胞分析,未来就其在模拟细胞微环境下的传感设计和适用性还需进行更加深入的研究。Liquid crystal(LC)biosensors develop based on highly sensitive responses of LC to interfacial properties and its inherent optical anisotropy,which show extraordinary application value in detecting and analyzing of biological samples.By decorating with a stimuli-responsive molecule,LC interface can sensitively respond to the presence of biological analytes,which directs the orientation of LC molecules at the LC interface.Further,the short-range interaction occurred at the LC interface can change the orientation of bulk LC phase and lead to a visual transfiguration through a polarized optical microscope(POM).LC biosensors does not need to label the target analyte,and easily transforms molecular events into detectable optical signals with a simple optical instrument.Because of the advantages of simplicity,sensitivity,efficiency,rapidity and low cost,LC biosensors have been widely used in the field of bioanalysis.Herein,this article briefly reviews the recent advances in LC biosensors for the analysis of small biomolecules,biomacromolecules and organism.In particular,we discuss the development and challenge of the emerging area where the LC biosensor is introduced to a microfluidic cell chip.LC biosensors have been successfully applied to cell analysis with complex and diverse interfacial phenomena.In the future,the design and usability of LC sensors in the simulated cell microenvironment need to be further studied.

关 键 词：液晶(LC) 生物传感 细胞分析 微流控 细胞微环境 

分 类 号：O657.7[理学—分析化学] Q26[理学—化学]