DEAH盒解旋酶15对转化生长因子-β_(1)诱导的呼吸道平滑肌细胞增殖和迁移的影响  

Effect of DEAH-box helicase 15 on the proliferation and migration of airway smooth muscle cells induced by transforming growth factor-β_(1)

作　　者：王静[1] 康媛洁 王贞[2] 唐青 陈瑶[2] WANG Jing;KANG Yuanjie;WANG Zhen;TANG Qing;CHEN Yao(Respiratory Asthma Center,Xi'an Children's Hospital,Xi'an 710003,Shaanxi Province,China;Department of Respiratory Medicine,Xi'an Children's Hospital,Xi'an 710003,Shaanxi Province,China)

机构地区：[1]西安市儿童医院呼吸哮喘中心,陕西西安710003 [2]西安市儿童医院呼吸二科,陕西西安710003

出　　处：《新乡医学院学报》2021年第5期413-417,共5页Journal of Xinxiang Medical University

摘　　要：目的探讨DEAH盒解旋酶15(DHX15)对转化生长因子-β_(1)(TGF-β_(1))诱导的呼吸道平滑肌细胞(ASMCs)增殖和迁移的影响。方法将TGF-β_(1)(20 mg·L^(-1))诱导的ASMCs随机分为对照组、阴性对照组和沉默组。阴性对照组和沉默组细胞分别转染control-siRNA和DHX15-siRNA质粒,对照组细胞不作处理。转染24 h后,采用实时荧光定量聚合酶链反应法检测各组细胞中DHX15 mRNA相对表达量,细胞计数试剂盒-8实验检测各组细胞增殖能力,Transwell小室实验检测各组细胞迁移能力,Western blot法检测各组细胞中磷酸化p65(p-p65)、磷酸化核因子抑制蛋白(p-IκB)、磷酸化c-Jun氨基末端激酶(p-JNK)、磷酸化p38(p-p38)蛋白的表达。结果对照组和阴性对照组ASMCs中DHX15 mRNA相对表达量、细胞增殖能力、细胞迁移能力比较差异无统计学意义(P>0.05);沉默组ASMCs中DHX15 mRNA相对表达量、细胞增殖能力、细胞迁移能力显著低于对照组和阴性对照组(P<0.05)。对照组和阴性对照组ASMCs中p-p65、p-IκB、p-JNK和p-p38蛋白相对表达量比较差异无统计学意义(P>0.05);沉默组ASMCs中p-p65、p-IκB、p-JNK和p-p38蛋白相对表达量均显著低于对照组和阴性对照组(P<0.05)。结论干扰DHX15表达可显著抑制TGF-β_(1)诱导的ASMCs的增殖和迁移能力,其机制可能与抑制核因子-κB和丝裂原活化蛋白激酶信号通路有关。Objective To investigate the effect of DEAH-box helicase 15(DHX15)on the proliferation and migration of airway smooth muscle cells(ASMCs)induced by transforming growth factor-β_(1)(TGF-β_(1)).Methods The ASMCs induced by TGF-β_(1)(20 mg·L^(-1))were randomly divided into control group,negative control group and silent group.The cells in the negative control group and the silence group were respectively transfected with control-siRNA and DHX15-siRNA plasmids,and the cells in the control group were not treated.After 24 hours of transfection,the relative expression level of DHX15 mRNA of the cells in each group was detected by real-time fluorescent quantitative polymerase chain reaction,the proliferation ability of cells in each group was detected by cell counting kit-8,the migration ability of cells in each group was detected by Transwell chamber experiment;the expressions of phosphorylated p65(p-p65),phosphorylated nuclear factor inhibitor protein(p-IκB),phosphorylated c-Jun N-terminalkinae(p-JNK)and phosphorylated p38(p-p38)protein of cells in each group were detected by Western blot.Results There was no significant difference in the relative expression level of DHX15 mRNA,cell proliferation ability and cell migration ability of ASMCs between the control group and negative control group(P>0.05).The relative expression level of DHX15 mRNA,cell proliferation ability and cell migration ability of ASMCs in the silence group were signific antly lower than those in the control group and the negative control group(P<0.05).There was no significant difference in the relative expression levels of p-p65,p-IκB,p-JNK and p-p38 of ASMCs induced by TGF-β_(1)between the control group and the negative control group(P>0.05).The relative expression levels of p-p65,p-IκB,p-JNK and p-p38 protein of the ASMCs in the silence group were significantly lower than those in the control group and the negative control group(P<0.05).Conclusion Interference with the expression of DHX15 can significantly inhibit the proliferation and migr

关 键 词：DEAH盒解旋酶15 核因子-ΚB信号通路 丝裂原活化蛋白激酶信号通路 哮喘 增殖 迁移 

分 类 号：R563[医药卫生—呼吸系统]