慢性酒精摄入对小鼠小脑分子层场电位的影响及NO信号通路机制  

Effects of chronic ethanol consumption on field potential of cerebellar molecular layer in mice and the NO signal mechanism

作　　者：董光辉 李文静 刘靓妍 林成权 崔松彪 Dong Guanghui;Li Wenjing;Liu Liangyan;Lin Chengquan;Cui Songbiao(Department of Neurology,Affiliated Hospital Yanbian University,Yanji 133000,China;Department of Anesthesiology,Affiliated Hospital Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学附属医院神经内科,延吉133000 [2]延边大学附属医院麻醉科,延吉133000

出　　处：《中华行为医学与脑科学杂志》2021年第5期391-396,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目(81860219)。

摘　　要：目的:研究慢性酒精摄入对小脑分子层感觉信息传递的影响,揭示慢性酒精中毒对小脑皮质感觉信息传递与信息整合影响的机制。方法:50只6~8周龄健康雄性ICR小鼠按照随机数字表法分为生理盐水组(对照组)和酒精摄入组(酒精组),每组25只,酒精组每日腹腔注射体积分数为20%酒精,对照组则注射同等剂量的生理盐水,所有小鼠每天腹腔注射一次,连续注射28 d。通过电生理技术运用膜片钳放大器及数据采集软件记录感觉刺激诱发酒精组及对照组小鼠小脑分子层场电位变化。采用Clampfit 10.3软件对电生理数据进行记录分析,采用SPSS 22.0软件对数据进行统计分析,使用配对t检验和单因素方差分析比较用药前后的差异。结果:给予吹风刺激后,酒精组小鼠的P1振幅较对照组显著增高[(121.3±3.5)%,(97.2±2.7)%;t=26.08,P<0.05),P1曲线下面积较对照组明显增大[(127.1±4.2)%,(102.2±3.5)%;t=22.95,P<0.05]。酒精组N1的平均振幅与对照组比较差异无统计学意义(P>0.05)。小鼠脑表面灌流一氧化氮合酶抑制剂L-NNA后,吹风刺激诱发的酒精组小鼠P1振幅(76.2±4.8)%较给药前(103.5±3.6)%显著降低(t=22.60,P<0.05),但洗脱后(101.5±4.6)%与给药前相比差异无统计学意义(t=1.70,P>0.05),P1曲线下面积(72.4±5.6)%较给药前(102.7±2.6)%显著降低(t=24.58,P<0.05),洗脱后(100.6±3.5)%与给药前差异无统计学意义(t=1.81,P>0.05)。小鼠脑表面灌流一氧化氮合酶抑制剂L-NNA后,对照组小鼠的P1振幅(104.3±1.6)%与给药后(102.2±5.6)%(t=1.84,P>0.05)及洗脱后(102.5±4.5)%(t=1.92,P>0.05)比较,均差异无统计学意义;P1曲线下面积(103.5±2.6)%较给药后(102.5±4.6)%(t=0.99,P>0.05)及洗脱后(101.9±3.7)%(t=1.81,P>0.05)差异无统计学意义。对照组小鼠脑表面灌流一氧化氮供体SNAP后,吹风刺激诱发分子层场电位P1振幅(128.2±3.4)%较给药前(103.5±2.6)%显著增加(t=28.89,P<0.05),洗脱后(105.4±4.2)%较给药前差�Objective To investigate the effect of chronic ethanol consumption on sensory information transmission in the cerebellar molecular layer and reveal the mechanism of chronic alcoholism on sensory information transmission and integration in the cerebellar cortex.Methods Fifty healthy male ICR mice aged 6-8 weeks were randomly divided into saline group(control group)and ethanol consumption group(alcohol group)according to the random number table,with 25 mice in each group.The mice in alcohol group were injected intraperitoneally with 20%ethanol daily,while the mice in control group were injected with the same dose of normal saline.All mice were injected intraperitoneally once a day for 28 days.Through electrophysiological technology,patch-clamp amplifier and data acquisition software were used to record the changes in cerebellar molecular layer field potential of mice in the alcohol group and control group induced by sensory stimulation.Clampfit 10.3 software was used to record and analyze the electrophysiological data.SPSS 22.0 software was used for statistical analysis.Paired t-test and one-way ANOVA were used to analyze the differences before and after treatment.Results After giving the stimulation of wind blowing,the amplitude of P1 in alcohol group was significantly higher than that in control group((121.31±3.5)%,(97.2±2.7)%;t=26.08,P<0.05),and the area under the P1 curve(AUC)of the alcohol group was significantly lower than that of the control group((127.1±4.2)%,(102.2±3.5)%;t=22.95,P<0.05).There was no significant difference in N1 amplitude between the two groups(P>0.05).When L-NNA,an inhibitor of nitric oxide synthase,was perfused into the brain surface of mice,the amplitude of P1 in alcohol group was significantly lower than that before administration((76.2±4.8)%,(103.5±3.6)%;t=22.60,P<0.05),but there was no difference of the amplitude of P1 before administration and after elution((101.5±4.6)%)(t=1.70,P>0.05).After the L-NNA was perfused,the AUC of P1 was significantly lower than that before administ

关 键 词：乙醇 小脑皮质 感觉刺激 一氧化氮 分子层 小鼠 

分 类 号：R595.6[医药卫生—内科学]