奶牛犊牛腹泻源大肠埃希氏菌耐药情况及LEE相关基因的检测  

Drug Resistance and Detection of LEE Related Genes in E.coli from Dairy Calves with Diarrhea

作　　者：高海慧 王建东 高小斐 王培柱 吴学青 厉龙 康晓冬 GAO Hai-hui;WANG Jian-dong;GAO Xiao-fei;WANG Pei-zhu;WU Xue-qing;LI Long;KANG Xiao-dong(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002,China;Animal Husbandry and Veterinary Station,Fanxue Town,Dingbian,Shaanxi 718699,China;Animal Husbandry and Veterinary Station,Biandangou Town,Litong,Wuzhong,Ningxia 751100,China;Animal Disease Prevention and Control Center of Huinong of Shizuishan City,Luoping,Ningxia 753200,China)

机构地区：[1]宁夏农林科学院动物科学研究所,宁夏银川750002 [2]陕西省榆林市定边县樊学镇畜牧兽医站,陕西定边718699 [3]宁夏吴忠市利通区扁担沟镇畜牧兽医工作站,宁夏吴忠751100 [4]宁夏石嘴山市惠农区动物疾病预防控制中心,宁夏平罗753200

出　　处：《动物医学进展》2021年第6期19-23,共5页Progress In Veterinary Medicine

基　　金：宁夏农林科学院农业科技自主创新项目(NKYQ-20-01)(NKYJ-20-12);农业高质量发展和生态保护科技创新示范专项(NGSB-2021-12-06);宁夏农林科学院平台提升项目(NKYP-19-14)。

摘　　要：为了明确宁夏地区奶牛犊牛腹泻源性大肠埃希氏菌的分离率、耐药情况和LEE毒力岛eaeA和ler基因的携带情况,采集宁夏5市16个规模化奶牛场1~2月龄犊牛病理性腹泻样本157份,采用微生物学方法培养、VITEK 2 Compact生化鉴定和PCR鉴定,应用PCR方法进行分离株LEE相关基因检测。结果分离鉴定到大肠埃希氏菌62株,对四环素耐药率为100%,对阿莫西林、氟苯尼考、哌拉西林等9种药物的耐药率大于90.00%,对阿米卡星较为敏感,耐药率为11.29%;LEE毒力岛eaeA基因的检出率为11.29%(7/62),ler基因的检出率为79.03%(49/62),二者同时检出率为3.23%(2/62)。结果表明,LEE的存在,可能是某些奶牛场犊牛腹泻大肠埃希氏菌病的原因之一,建议临床治疗犊牛腹泻大肠埃希氏菌病可以首选阿米卡星。The purpose of this study was to determine the isolation rate,drug resistance and the carrying of eaeA and ler LEE related genes of E.coli from diarrhea calves(1-2 months)in Ningxia.157 anal swab samples were collected from 16 scale dairy farms in 5 cities cultured by microbiology,identified by VITEK 2 compact biochemistry and PCR methods.LEE-related genes were detected by PCR.The results showed that 62 strains of E.coli were isolated,The drug resistance rate of the isolates to tetracycline was 100%,to amoxicillin,florfenicol,pipracil and other nine kinds of drugs were>90%,and sensitive to amikacin,the resistance rate was 11.29%.Detection rate of eaeA and ler genes were 11.29%(7/62)and 79.03%(49/62)respectively,both simutaneous detection rate was 3.23%(2/62).In conclusion,one of the reasons caused calves diarrhea by E.coli was carrying LEE,clinical treatment of diarrhea calves caused by E.coli can choose amikacin first.

关 键 词：奶牛 犊牛腹泻 大肠埃希氏菌 耐药 肠细胞脱落位点 

分 类 号：S823.91[农业科学—畜牧学] S858.23[农业科学—畜牧兽医]